Stable transformation of maize: The impact of feeder cells on protoplast growth and transformation efficiency
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Plant Cell Reports v. 8, no. 5, p. 292-295615170Summary: Protoplast cultures of the F1 hybrid A188 X Black Mexican Sweet were transformed using 2 plasmids, pPUR carrying genes coding for beta-glucuronidase (GUS) and pKAN carryong the gene neo neomycin resistance, by either electroporation or PEG [polyethylene glycol] treatment. Total GUS activity, measured 2 days after transformation, was 5-fold higher in protoplasts cultured on feeder cells compared to those grown in the absence of feeder cells. Since the specific activity of GUS was only slightly higher in the transformed protoplasts plated over feeder cells, the stimulation in transient gene expression resulted mainly from the improved environment
| Item type | Current library | Collection | Call number | Copy number | Status | Barcode | |
|---|---|---|---|---|---|---|---|
| Article | CIMMYT Knowledge Center: John Woolston Library | Reprints Collection | REP-4881 (Browse shelf(Opens below)) | 1 | Available | 615170 |
Illustrations, tables, graphs, references p. 295
Protoplast cultures of the F1 hybrid A188 X Black Mexican Sweet were transformed using 2 plasmids, pPUR carrying genes coding for beta-glucuronidase (GUS) and pKAN carryong the gene neo neomycin resistance, by either electroporation or PEG [polyethylene glycol] treatment. Total GUS activity, measured 2 days after transformation, was 5-fold higher in protoplasts cultured on feeder cells compared to those grown in the absence of feeder cells. Since the specific activity of GUS was only slightly higher in the transformed protoplasts plated over feeder cells, the stimulation in transient gene expression resulted mainly from the improved environment
English
Springer
Reprints Collection
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