Stable transformation of maize:
Lyznik, L.A.
Stable transformation of maize: The impact of feeder cells on protoplast growth and transformation efficiency - 1989 - Printed
Illustrations, tables, graphs, references p. 295
Protoplast cultures of the F1 hybrid A188 X Black Mexican Sweet were transformed using 2 plasmids, pPUR carrying genes coding for beta-glucuronidase (GUS) and pKAN carryong the gene neo neomycin resistance, by either electroporation or PEG [polyethylene glycol] treatment. Total GUS activity, measured 2 days after transformation, was 5-fold higher in protoplasts cultured on feeder cells compared to those grown in the absence of feeder cells. Since the specific activity of GUS was only slightly higher in the transformed protoplasts plated over feeder cells, the stimulation in transient gene expression resulted mainly from the improved environment
English
Antibiotics
Cell culture
Genes
Genetic transformation
Protoplasts
Zea mays
Stable transformation of maize: The impact of feeder cells on protoplast growth and transformation efficiency - 1989 - Printed
Illustrations, tables, graphs, references p. 295
Protoplast cultures of the F1 hybrid A188 X Black Mexican Sweet were transformed using 2 plasmids, pPUR carrying genes coding for beta-glucuronidase (GUS) and pKAN carryong the gene neo neomycin resistance, by either electroporation or PEG [polyethylene glycol] treatment. Total GUS activity, measured 2 days after transformation, was 5-fold higher in protoplasts cultured on feeder cells compared to those grown in the absence of feeder cells. Since the specific activity of GUS was only slightly higher in the transformed protoplasts plated over feeder cells, the stimulation in transient gene expression resulted mainly from the improved environment
English
Antibiotics
Cell culture
Genes
Genetic transformation
Protoplasts
Zea mays