Knowledge Center Catalog

Stable transformation of maize: (Record no. 11896)

MARC details
000 -LEADER
fixed length control field 01740nab a22004097a 4500
001 - CONTROL NUMBER
control field G28760
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170719154933.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title En
043 ## - GEOGRAPHIC AREA CODE
Geographic area code DE
072 #0 - SUBJECT CATEGORY CODE
Subject category code F30
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) REP-4881
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Lyznik, L.A.
245 00 - TITLE STATEMENT
Title Stable transformation of maize:
Remainder of title The impact of feeder cells on protoplast growth and transformation efficiency
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 1989
340 ## - PHYSICAL MEDIUM
Material base and configuration Printed
500 ## - GENERAL NOTE
General note Illustrations, tables, graphs, references p. 295
520 ## - SUMMARY, ETC.
Summary, etc. Protoplast cultures of the F1 hybrid A188 X Black Mexican Sweet were transformed using 2 plasmids, pPUR carrying genes coding for beta-glucuronidase (GUS) and pKAN carryong the gene neo neomycin resistance, by either electroporation or PEG [polyethylene glycol] treatment. Total GUS activity, measured 2 days after transformation, was 5-fold higher in protoplasts cultured on feeder cells compared to those grown in the absence of feeder cells. Since the specific activity of GUS was only slightly higher in the transformed protoplasts plated over feeder cells, the stimulation in transient gene expression resulted mainly from the improved environment
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation Springer
595 ## - COLLECTION
Collection Reprints Collection
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Antibiotics
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Cell culture
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Genes
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Genetic transformation
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Protoplasts
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 1314
Topical term or geographic name as entry element Zea mays
Miscellaneous information AGROVOC
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Chang, K.L.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Grimes, H.D.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hodges, T.K.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kamo, K.K.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Ryan, R,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Plant Cell Reports
Note 615170
Related parts v. 8, no. 5, p. 292-295
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Holdings
Date last seen Total Checkouts Full call number Barcode Copy number Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
02/10/2015   REP-4881 615170 1 02/10/2015 Article Not Lost Dewey Decimal Classification     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 02/10/2015

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