Classification of heterotic groups among maize germplasm in China using molecular markers
Material type:
TextPublication details: Mexico, DF (Mexico) CIMMYT : 2004Description: p. 43-49ISBN: - 970-648-116-8
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Publications Collection | Look under series title (Browse shelf(Opens below)) | 1 | Available | E630601 |
Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: CIMMYT Publications Collection Close shelf browser (Hides shelf browser)
| Look under series title Resistance of Bt maize to the asian corn borer (Lepidoptera: Pyralidae) in China | Look under series title Germplasm basis and QTL mapping for resistance to SCMV in chinese maize | Look under series title Construction of molecular marker linkage map and genetic analysis of plant height in maize | Look under series title Classification of heterotic groups among maize germplasm in China using molecular markers | Look under series title Biolistic introduction of a modified CpTI (SCK) gene into an elite maize inbread line | Look under series title Heterotic groups and exploitation of heterosis--methodology, strategy, and use in hybrid maize breeding in China | Look under series title Application of molecular marker technologies for maize improvement in India - present status and prospects |
Classification of heterotic groups is essential for maize breeding. RFLP has provided a powerful tool to assign maize inbred lines into heterotic groups. In this study, 45 inbred lines that are used widely in China, besides four lines from USA were chosen for RFLP analysis. Fufty-four RFLP core markers covering the 10 chromosomes of maize were used. The total DNA of each sample was digested with EcoR I, BamH I and Hind III. A total of 860 bands were detected among the 45 inbred lines based on RFLP analysis, mapping to 212 loci. A number of allele at each locus ranged from 2 to 9 with an average of 4.06. In total, 45 inbred lines were classified into six heterotic groups according to RFLPs. Three heterotic groups, including Mo 17, B73 and Oh43 respectively, represented the U.S. heterotic groups. Twenty-one inbred lines, most of which were derived from the Chinese local germplasm, were classified into two heterotic groups, indicating that the local germplasm was different from U.S. germplasm at the molecular level, and played an important role in maize hybrid production in China. Two inbred lines from the tropical germplasm were assigned to the same group. These results provided useful information to understand maize heterotic groups and heterotic patterns in China.
English
0501|AGRIS 0501|AL-Maize Program
Juan Carlos Mendieta
CIMMYT Publications Collection