Classification of heterotic groups among maize germplasm in China using molecular markers
Yi-qin, H.
Classification of heterotic groups among maize germplasm in China using molecular markers - Mexico, DF (Mexico) CIMMYT : 2004 - p. 43-49 - Printed
Classification of heterotic groups is essential for maize breeding. RFLP has provided a powerful tool to assign maize inbred lines into heterotic groups. In this study, 45 inbred lines that are used widely in China, besides four lines from USA were chosen for RFLP analysis. Fufty-four RFLP core markers covering the 10 chromosomes of maize were used. The total DNA of each sample was digested with EcoR I, BamH I and Hind III. A total of 860 bands were detected among the 45 inbred lines based on RFLP analysis, mapping to 212 loci. A number of allele at each locus ranged from 2 to 9 with an average of 4.06. In total, 45 inbred lines were classified into six heterotic groups according to RFLPs. Three heterotic groups, including Mo 17, B73 and Oh43 respectively, represented the U.S. heterotic groups. Twenty-one inbred lines, most of which were derived from the Chinese local germplasm, were classified into two heterotic groups, indicating that the local germplasm was different from U.S. germplasm at the molecular level, and played an important role in maize hybrid production in China. Two inbred lines from the tropical germplasm were assigned to the same group. These results provided useful information to understand maize heterotic groups and heterotic patterns in China.
English
970-648-116-8
Chromosomes
Crop yield
Germplasm
Heterotis
Hybridization
Inbred lines
Maize
Plant breeding
CIMMYT
Classification of heterotic groups among maize germplasm in China using molecular markers - Mexico, DF (Mexico) CIMMYT : 2004 - p. 43-49 - Printed
Classification of heterotic groups is essential for maize breeding. RFLP has provided a powerful tool to assign maize inbred lines into heterotic groups. In this study, 45 inbred lines that are used widely in China, besides four lines from USA were chosen for RFLP analysis. Fufty-four RFLP core markers covering the 10 chromosomes of maize were used. The total DNA of each sample was digested with EcoR I, BamH I and Hind III. A total of 860 bands were detected among the 45 inbred lines based on RFLP analysis, mapping to 212 loci. A number of allele at each locus ranged from 2 to 9 with an average of 4.06. In total, 45 inbred lines were classified into six heterotic groups according to RFLPs. Three heterotic groups, including Mo 17, B73 and Oh43 respectively, represented the U.S. heterotic groups. Twenty-one inbred lines, most of which were derived from the Chinese local germplasm, were classified into two heterotic groups, indicating that the local germplasm was different from U.S. germplasm at the molecular level, and played an important role in maize hybrid production in China. Two inbred lines from the tropical germplasm were assigned to the same group. These results provided useful information to understand maize heterotic groups and heterotic patterns in China.
English
970-648-116-8
Chromosomes
Crop yield
Germplasm
Heterotis
Hybridization
Inbred lines
Maize
Plant breeding
CIMMYT