Knowledge Center Catalog

Marker assisted selection for quality protein maize

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT : 2003Description: p. 140-141Subject(s): DDC classification:
  • 631.53 BOO
Summary: Conventional maize is poor in quality protein, especially lysine and tryptophan. Quality protein maize (QPM) lines have been produced by the introgression of the opaque-2 gene which regulates the expression of zein, and the modifier genes for hard endosperm. The content of lysine and tryptophan is, therefore, greatly improved. Compared with conventional methods, marker assisted selection allows the identification of QPM individuals in an early breeding cycle by identifying the presence or absence of 02 prior to pollination, and the breeders can distinguish between homozygous recessive plants o2o2 and heterozygous plants o2o2. In order to reduce the cost of field evaluation and facilitate the QPM breeding program, simple sequence repeat (SSR) markers were employed in the QPM breeding program to transfer o2 alleles and their associated modifier genes into the normal elite lines (NEL).
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Publications Collection 631.53 BOO (Browse shelf(Opens below)) 1 Available 1R632399
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Conventional maize is poor in quality protein, especially lysine and tryptophan. Quality protein maize (QPM) lines have been produced by the introgression of the opaque-2 gene which regulates the expression of zein, and the modifier genes for hard endosperm. The content of lysine and tryptophan is, therefore, greatly improved. Compared with conventional methods, marker assisted selection allows the identification of QPM individuals in an early breeding cycle by identifying the presence or absence of 02 prior to pollination, and the breeders can distinguish between homozygous recessive plants o2o2 and heterozygous plants o2o2. In order to reduce the cost of field evaluation and facilitate the QPM breeding program, simple sequence repeat (SSR) markers were employed in the QPM breeding program to transfer o2 alleles and their associated modifier genes into the normal elite lines (NEL).

English

0309|AGRIS 0301|AL-Maize Program

Juan Carlos Mendieta

CIMMYT Publications Collection


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