A new identification system for South African wheat cultivars based on HMW and LMW gluten subunit composition
Material type:
TextPublication details: Addis Ababa (Ethiopia) CIMMYT : 1999ISBN: - 92-9146-058-3
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Plant breeders ' rights are becoming more and more important, not only in South Africa, but also in the rest of the world. In South Africa to date, mainly the high molecular weight (HMW) glutenin subunits (GS) have been used for cultivar identification. Unfortunately, many commercial cultivars have exactly the same HMW glutenin subunits. The research on low molecular weight (LMW) subunits was limited in the past due to their inadequate fractionation in SDS-P AGE. After the introduction of a simplified one-step, one-dimensional SDS-PAGE procedure by Singh et al. (1991) the analysis of a large number of samples in a gliadin-free background was possible. The aim of this study was to determine an identification system for South African wheat cultivars based on their HMW and LMW glutenin subunit composition. It was not possible to distinguish between all the cultivars tested using the HMW -GS as many of the cultivars had the same banding patterns. With the LMW -GS it was possible to distinguish clearly between all the cultivars tested. The advantages and disadvantages of both techniques are discussed.
English
0007|AGRIS 0101|AL-Wheat Program
Jose Juan Caballero
CIMMYT Publications Collection