Knowledge Center Catalog

A new identification system for South African wheat cultivars based on HMW and LMW gluten subunit composition

By: Contributor(s): Material type: TextTextPublication details: Addis Ababa (Ethiopia) CIMMYT : 1999ISBN:
  • 92-9146-058-3
Subject(s): Summary: Plant breeders ' rights are becoming more and more important, not only in South Africa, but also in the rest of the world. In South Africa to date, mainly the high molecular weight (HMW) glutenin subunits (GS) have been used for cultivar identification. Unfortunately, many commercial cultivars have exactly the same HMW glutenin subunits. The research on low molecular weight (LMW) subunits was limited in the past due to their inadequate fractionation in SDS-P AGE. After the introduction of a simplified one-step, one-dimensional SDS-PAGE procedure by Singh et al. (1991) the analysis of a large number of samples in a gliadin-free background was possible. The aim of this study was to determine an identification system for South African wheat cultivars based on their HMW and LMW glutenin subunit composition. It was not possible to distinguish between all the cultivars tested using the HMW -GS as many of the cultivars had the same banding patterns. With the LMW -GS it was possible to distinguish clearly between all the cultivars tested. The advantages and disadvantages of both techniques are discussed.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Publications Collection Look under series title (Browse shelf(Opens below)) 1 Available 2E629146
Total holds: 0

Plant breeders ' rights are becoming more and more important, not only in South Africa, but also in the rest of the world. In South Africa to date, mainly the high molecular weight (HMW) glutenin subunits (GS) have been used for cultivar identification. Unfortunately, many commercial cultivars have exactly the same HMW glutenin subunits. The research on low molecular weight (LMW) subunits was limited in the past due to their inadequate fractionation in SDS-P AGE. After the introduction of a simplified one-step, one-dimensional SDS-PAGE procedure by Singh et al. (1991) the analysis of a large number of samples in a gliadin-free background was possible. The aim of this study was to determine an identification system for South African wheat cultivars based on their HMW and LMW glutenin subunit composition. It was not possible to distinguish between all the cultivars tested using the HMW -GS as many of the cultivars had the same banding patterns. With the LMW -GS it was possible to distinguish clearly between all the cultivars tested. The advantages and disadvantages of both techniques are discussed.

English

0007|AGRIS 0101|AL-Wheat Program

Jose Juan Caballero

CIMMYT Publications Collection


International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org