Mating type-specific PCR primers for Stagonospora nodorum field studies
Material type:
TextPublication details: Mexico, DF (Mexico) CIMMYT : 1999ISBN: - 970-648-035-8
- 632.4 GIN
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Publications Collection | 632.4 GIN (Browse shelf(Opens below)) | 1 | Available | X628903 |
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Conserved regions of the mating type genes (MAT) in Cochliobolus heterostrophus, Mycosphaerella zeae-maydis, and Alternaria alternata were used to design primers to identify the mating type genes and thus assign mating types to Stagonospora nodorum. These primers successfully distinguished between mating types of S. nodorum isolates that had been identified through traditional crosses. The primers were used to screen a small sample of S. nodorum isolates from New York, thus revealing the presence of both mating types. This efficient method of identifying mating types may help determine the role of sexual recombination in the epidemiology of S. nodorum, as well as elucidate phylogenetic relationships among related species.
English
9910|AGRIS 0001
Jose Juan Caballero
CIMMYT Publications Collection