Knowledge Center Catalog

Mating type-specific PCR primers for Stagonospora nodorum field studies

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT : 1999ISBN:
  • 970-648-035-8
Subject(s): DDC classification:
  • 632.4 GIN
Summary: Conserved regions of the mating type genes (MAT) in Cochliobolus heterostrophus, Mycosphaerella zeae-maydis, and Alternaria alternata were used to design primers to identify the mating type genes and thus assign mating types to Stagonospora nodorum. These primers successfully distinguished between mating types of S. nodorum isolates that had been identified through traditional crosses. The primers were used to screen a small sample of S. nodorum isolates from New York, thus revealing the presence of both mating types. This efficient method of identifying mating types may help determine the role of sexual recombination in the epidemiology of S. nodorum, as well as elucidate phylogenetic relationships among related species.
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Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Publications Collection 632.4 GIN (Browse shelf(Opens below)) 1 Available X628903
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Conserved regions of the mating type genes (MAT) in Cochliobolus heterostrophus, Mycosphaerella zeae-maydis, and Alternaria alternata were used to design primers to identify the mating type genes and thus assign mating types to Stagonospora nodorum. These primers successfully distinguished between mating types of S. nodorum isolates that had been identified through traditional crosses. The primers were used to screen a small sample of S. nodorum isolates from New York, thus revealing the presence of both mating types. This efficient method of identifying mating types may help determine the role of sexual recombination in the epidemiology of S. nodorum, as well as elucidate phylogenetic relationships among related species.

English

9910|AGRIS 0001

Jose Juan Caballero

CIMMYT Publications Collection


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