Mating type-specific PCR primers for Stagonospora nodorum field studies
Bennett, R.S.
Mating type-specific PCR primers for Stagonospora nodorum field studies - Mexico, DF (Mexico) CIMMYT : 1999 - Printed
Conserved regions of the mating type genes (MAT) in Cochliobolus heterostrophus, Mycosphaerella zeae-maydis, and Alternaria alternata were used to design primers to identify the mating type genes and thus assign mating types to Stagonospora nodorum. These primers successfully distinguished between mating types of S. nodorum isolates that had been identified through traditional crosses. The primers were used to screen a small sample of S. nodorum isolates from New York, thus revealing the presence of both mating types. This efficient method of identifying mating types may help determine the role of sexual recombination in the epidemiology of S. nodorum, as well as elucidate phylogenetic relationships among related species.
English
970-648-035-8
DNA
Genetic markers
Hybridization
Mycosphaerella
Plant diseases
CIMMYT
632.4 / GIN
Mating type-specific PCR primers for Stagonospora nodorum field studies - Mexico, DF (Mexico) CIMMYT : 1999 - Printed
Conserved regions of the mating type genes (MAT) in Cochliobolus heterostrophus, Mycosphaerella zeae-maydis, and Alternaria alternata were used to design primers to identify the mating type genes and thus assign mating types to Stagonospora nodorum. These primers successfully distinguished between mating types of S. nodorum isolates that had been identified through traditional crosses. The primers were used to screen a small sample of S. nodorum isolates from New York, thus revealing the presence of both mating types. This efficient method of identifying mating types may help determine the role of sexual recombination in the epidemiology of S. nodorum, as well as elucidate phylogenetic relationships among related species.
English
970-648-035-8
DNA
Genetic markers
Hybridization
Mycosphaerella
Plant diseases
CIMMYT
632.4 / GIN