Stability of ELISA activity in frozen samples of barley yellow dwarf virus-infected leaf and leaf extracts
Material type:
TextLanguage: En Publication details: 1990ISBN: - 968-6127-39
- 91-013910
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Reprint | CIMMYT Knowledge Center: John Woolston Library | AGRIS Collection | 91-013910 (Browse shelf(Opens below)) | 1 | Available | 91-013910 |
Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: AGRIS Collection Close shelf browser (Hides shelf browser)
10 ref
When replicate samples of barley yellow dwarf virus-infected intact oat (Avena sativa L.) leaf, leaf extracts in phosphate buffer, or chloroform-clarified leaf extracts, were stored frozen at -20 deg C, -80 deg C, or in liquid nitrogen, their enzyme-linked immunosorbent assay (ELISA) activities fluctuated over time and according to the virus isolate involved. With the Purdue (P)-PAV isolate, no significant decrease of ELISA activity was noted with leaf, crude leaf extracts, or clarified extracts stored for up to 4 weeks at -20 deg C, -80 deg C, or in liquid nitrogen, except that clarified extracts stored at -20 deg C deteriorated after 1 week.
English
World Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD
AGRIS Collection