Stability of ELISA activity in frozen samples of barley yellow dwarf virus-infected leaf and leaf extracts
Nazare Pereira, A.M.
Stability of ELISA activity in frozen samples of barley yellow dwarf virus-infected leaf and leaf extracts - 1990 - Printed
10 ref
When replicate samples of barley yellow dwarf virus-infected intact oat (Avena sativa L.) leaf, leaf extracts in phosphate buffer, or chloroform-clarified leaf extracts, were stored frozen at -20 deg C, -80 deg C, or in liquid nitrogen, their enzyme-linked immunosorbent assay (ELISA) activities fluctuated over time and according to the virus isolate involved. With the Purdue (P)-PAV isolate, no significant decrease of ELISA activity was noted with leaf, crude leaf extracts, or clarified extracts stored for up to 4 weeks at -20 deg C, -80 deg C, or in liquid nitrogen, except that clarified extracts stored at -20 deg C deteriorated after 1 week.
English
968-6127-39
Avena
Chemicophysical properties
Developmental stages
Freezants
Gramineae
Immunoenzyme techniques
Immunological techniques
Inorganic acid salts
Luteoviruses
Plant anatomy
Plant diseases
Plant vegetative organs
Plant viruses
Processing aids
Salts
Viruses
91-013910
Stability of ELISA activity in frozen samples of barley yellow dwarf virus-infected leaf and leaf extracts - 1990 - Printed
10 ref
When replicate samples of barley yellow dwarf virus-infected intact oat (Avena sativa L.) leaf, leaf extracts in phosphate buffer, or chloroform-clarified leaf extracts, were stored frozen at -20 deg C, -80 deg C, or in liquid nitrogen, their enzyme-linked immunosorbent assay (ELISA) activities fluctuated over time and according to the virus isolate involved. With the Purdue (P)-PAV isolate, no significant decrease of ELISA activity was noted with leaf, crude leaf extracts, or clarified extracts stored for up to 4 weeks at -20 deg C, -80 deg C, or in liquid nitrogen, except that clarified extracts stored at -20 deg C deteriorated after 1 week.
English
968-6127-39
Avena
Chemicophysical properties
Developmental stages
Freezants
Gramineae
Immunoenzyme techniques
Immunological techniques
Inorganic acid salts
Luteoviruses
Plant anatomy
Plant diseases
Plant vegetative organs
Plant viruses
Processing aids
Salts
Viruses
91-013910