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The estimation of the available lysine in animal-protein foods

By: Material type: ArticleArticleLanguage: English Publication details: 1960. United Kingdom : Portland Press,ISSN:
  • 0264-6021
  • 1470-8728 (Online)
Subject(s): In: Biochemical Journal v. 77, no. 3, p. 604-610Summary: This paper describes the development of a procedure for the estimation in foods of the lysine units whose <-amino groups will undergo the Sanger reaction with fluorodinitrobenzene. There is reason to think that those lysine units in the proteins of heat-processed foods whose <-amino groups are bound to other groups, and so are unable to react, are likely also to be nutritionally 'unavailable' (Eldred & Rodney, 1946; Lea & Hannan, 1950; Henry & Kon, 1950). As a consequence of this, methods of analysis for lysine that begin with acid hydrolysis have been found to overvalue foods as sources of this amino acid.
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This paper describes the development of a procedure for the estimation in foods of the lysine units whose <-amino groups will undergo the Sanger reaction with fluorodinitrobenzene. There is reason to think that those lysine units in the proteins of heat-processed foods whose <-amino groups are bound to other groups, and so are unable to react, are likely also to be nutritionally 'unavailable' (Eldred & Rodney, 1946; Lea & Hannan, 1950; Henry & Kon, 1950). As a consequence of this, methods of analysis for lysine that begin with acid hydrolysis have been found to overvalue foods as sources of this amino acid.

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