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Mitotic activity in protoplasts isolated from Sorghum bicolor leaves

By: Contributor(s): Material type: ArticleArticleLanguage: En Publication details: 1981Subject(s): DDC classification:
  • 82-765572
 In: Plant Science Letters v. 23, no. 1, p. 11-1682-765572Summary: Factors affecting viability and mitotic activity of mesophyll protoplasts of Sorghum bicolor have been investigated. Protoplasts were isolated from leaves of 7-day-old seedlings which had been kept in continuous darkness for 3 days prior to enzymatic digestion. Subsequent incubation of these protoplasts in a medium conditioned by prior growth of sorghum shoot callus resulted in over 90 per cent of the original population remaining viable after 1 day and 5.2 per cent undergoing nuclear division after 17 days. Also, [('3)H]-thymidine incorporation and autoradiography confirmed that DNA synthesis was occurred in these protoplasts. Nuclear division
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7 ref.; Summary (En)

Factors affecting viability and mitotic activity of mesophyll protoplasts of Sorghum bicolor have been investigated. Protoplasts were isolated from leaves of 7-day-old seedlings which had been kept in continuous darkness for 3 days prior to enzymatic digestion. Subsequent incubation of these protoplasts in a medium conditioned by prior growth of sorghum shoot callus resulted in over 90 per cent of the original population remaining viable after 1 day and 5.2 per cent undergoing nuclear division after 17 days. Also, [('3)H]-thymidine incorporation and autoradiography confirmed that DNA synthesis was occurred in these protoplasts. Nuclear division

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