Mitotic activity in protoplasts isolated from Sorghum bicolor leaves (Record no. 14588)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01397nab a22003017a 4500 |
| 001 - CONTROL NUMBER | |
| control field | G49866 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 121211b |||p||p||||||| |z||| | |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | En |
| 043 ## - GEOGRAPHIC AREA CODE | |
| Geographic area code | NL |
| 072 #0 - SUBJECT CATEGORY CODE | |
| Subject category code | F30 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 82-765572 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Karunaratne, S.M. |
| 245 00 - TITLE STATEMENT | |
| Title | Mitotic activity in protoplasts isolated from Sorghum bicolor leaves |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Date of publication, distribution, etc. | 1981 |
| 340 ## - PHYSICAL MEDIUM | |
| Material base and configuration | Printed |
| 500 ## - GENERAL NOTE | |
| General note | 7 ref.; Summary (En) |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Factors affecting viability and mitotic activity of mesophyll protoplasts of Sorghum bicolor have been investigated. Protoplasts were isolated from leaves of 7-day-old seedlings which had been kept in continuous darkness for 3 days prior to enzymatic digestion. Subsequent incubation of these protoplasts in a medium conditioned by prior growth of sorghum shoot callus resulted in over 90 per cent of the original population remaining viable after 1 day and 5.2 per cent undergoing nuclear division after 17 days. Also, [('3)H]-thymidine incorporation and autoradiography confirmed that DNA synthesis was occurred in these protoplasts. Nuclear division |
| 546 ## - LANGUAGE NOTE | |
| Language note | English |
| 591 ## - CATALOGING NOTES | |
| Affiliation | COMOD |
| 595 ## - COLLECTION | |
| Collection | AGRIS Collection |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Sorghums sorghum spp |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant breeding |
| Miscellaneous information | AGROVOC |
| Source of heading or term | |
| 9 (RLIN) | 1203 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Scott, K.J., |
| Relator term | coaut. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Plant Science Letters |
| Note | 82-765572 |
| Related parts | v. 23, no. 1, p. 11-16 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Date last seen | Total Checkouts | Full call number | Barcode | Copy number | Price effective from | Koha item type | Lost status | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 02/10/2015 | 82-765572 | 82-765572 | 1 | 02/10/2015 | Article | Not Lost | AGRIS Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 02/10/2015 |