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Amino acid sequences of hordein polypeptides [barley, prolamin, carboxy-terminus]

By: Contributor(s): Material type: ArticleArticleLanguage: En Publication details: 1980ISSN:
  • 0105-1938
Subject(s): DDC classification:
  • 81-633120
 In: Carlsberg Research Communications v. 45, no. 2, p. 143-14881-633120Summary: Total hordein and purified hordein polypeptides B1 and C2 have been analyzed by carboxypeptidase Y digestion and sequential Edman degradation. The carboxypeptidase Y-catalyzed release of amino acids reveals the sequence (Ile. Ser)-Ser-Met-Val-COOH for the carboxy terminus of the C2 polypeptide. The same amino acids, with an additional glycine, were released from a total hordein preparation. Glycine has previously been found in the carboxyterminal sequence -Gly-Val-COOH of the B1 polypeptide. The B1 polypeptide was blocked to Edman degradation. The C2 polypeptide yielded a single amino acid sequence: H(,2)N-Arg-Gln-Leu-Asn-Pro-Ser-Ser-Gln-Glu-Leu- X
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Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library AGRIS Collection 81-633120 (Browse shelf(Opens below)) 1 Available 81-633120
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25 ref. Summary (En)

Total hordein and purified hordein polypeptides B1 and C2 have been analyzed by carboxypeptidase Y digestion and sequential Edman degradation. The carboxypeptidase Y-catalyzed release of amino acids reveals the sequence (Ile. Ser)-Ser-Met-Val-COOH for the carboxy terminus of the C2 polypeptide. The same amino acids, with an additional glycine, were released from a total hordein preparation. Glycine has previously been found in the carboxyterminal sequence -Gly-Val-COOH of the B1 polypeptide. The B1 polypeptide was blocked to Edman degradation. The C2 polypeptide yielded a single amino acid sequence: H(,2)N-Arg-Gln-Leu-Asn-Pro-Ser-Ser-Gln-Glu-Leu- X

English

Carlsberg Lab., Copenhagen (Denmark)|COMOD

AGRIS Collection


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