Amino acid sequences of hordein polypeptides [barley, prolamin, carboxy-terminus]
Schmitt, J.M.
Amino acid sequences of hordein polypeptides [barley, prolamin, carboxy-terminus] - 1980 - Printed
25 ref. Summary (En)
Total hordein and purified hordein polypeptides B1 and C2 have been analyzed by carboxypeptidase Y digestion and sequential Edman degradation. The carboxypeptidase Y-catalyzed release of amino acids reveals the sequence (Ile. Ser)-Ser-Met-Val-COOH for the carboxy terminus of the C2 polypeptide. The same amino acids, with an additional glycine, were released from a total hordein preparation. Glycine has previously been found in the carboxyterminal sequence -Gly-Val-COOH of the B1 polypeptide. The B1 polypeptide was blocked to Edman degradation. The C2 polypeptide yielded a single amino acid sequence: H(,2)N-Arg-Gln-Leu-Asn-Pro-Ser-Ser-Gln-Glu-Leu- X
English
0105-1938
Barley hordeum spp.
Proteins, amides and amino acids
Proximate composition
Plant breeding
81-633120
Amino acid sequences of hordein polypeptides [barley, prolamin, carboxy-terminus] - 1980 - Printed
25 ref. Summary (En)
Total hordein and purified hordein polypeptides B1 and C2 have been analyzed by carboxypeptidase Y digestion and sequential Edman degradation. The carboxypeptidase Y-catalyzed release of amino acids reveals the sequence (Ile. Ser)-Ser-Met-Val-COOH for the carboxy terminus of the C2 polypeptide. The same amino acids, with an additional glycine, were released from a total hordein preparation. Glycine has previously been found in the carboxyterminal sequence -Gly-Val-COOH of the B1 polypeptide. The B1 polypeptide was blocked to Edman degradation. The C2 polypeptide yielded a single amino acid sequence: H(,2)N-Arg-Gln-Leu-Asn-Pro-Ser-Ser-Gln-Glu-Leu- X
English
0105-1938
Barley hordeum spp.
Proteins, amides and amino acids
Proximate composition
Plant breeding
81-633120