Knowledge Center Catalog

Local cover image
Local cover image

Allele mining in the Yellow1 (Y1) gene influencing carotenoid biosynthesis in maize

By: Contributor(s): Material type: ArticleArticleLanguage: English Publication details: Mexico, DF (Mexico) : CIMMYT, 2010.ISBN:
  • 978-979-1159-41-8
Subject(s): In: Asian Regional Maize Workshop, 10. Maize for Asia - Emerging Trends and Technologies; Proceedings of The Asian Regional Maize Workshop; Makassar Indonesia; 20-23 Oct. 2008 p. 224-227Summary: Carotenoids are a class of fat-soluble antioxidant vitamin compounds that provide health benefits to animals and humans. Four carotenoid compounds are predominant in maize grain: a- carotene, a-cryptoxanthin, zeaxanthin and lutein. The present study pertains to an analysis of 24 maize inbred lines out of an association mapping panel for nucleotide diversity in the Yellow1 (Y1) gene which regulates an important step in carotenoid biosynthesis in maize kernels. The 5 untranslated region (UTR) of the Y1 gene was specifically targeted for study since an earlier analysis had revealed that most of the variation associated with this gene is likely due to cis-acting regulatory sequences. Three gene-specific primer pairs for PCR were designed using PRIMER3 software based on the gene sequence available in the public domain. The PCR products were purified and quantified prior to DNA sequencing. Sequence ambiguities, if any, were resolved by repeated sequencing (at least three replicates) from both ends. All the sequences were analyzed using different programs of Bioedit software, leading to identification of several SNPs and indels. A total of 15 sequence variations have been found in the present study with a frequency of 1 SNP per 87 bp. Haplotyping and intensive phenotyping of the selected inbred lines is in progress for identification of favorable allelic variants for carotenoid biosynthesis in maize.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)

Carotenoids are a class of fat-soluble antioxidant vitamin compounds that provide health benefits to animals and humans. Four carotenoid compounds are predominant in maize grain: a- carotene, a-cryptoxanthin, zeaxanthin and lutein. The present study pertains to an analysis of 24 maize inbred lines out of an association mapping panel for nucleotide diversity in the Yellow1 (Y1) gene which regulates an important step in carotenoid biosynthesis in maize kernels. The 5 untranslated region (UTR) of the Y1 gene was specifically targeted for study since an earlier analysis had revealed that most of the variation associated with this gene is likely due to cis-acting regulatory sequences. Three gene-specific primer pairs for PCR were designed using PRIMER3 software based on the gene sequence available in the public domain. The PCR products were purified and quantified prior to DNA sequencing. Sequence ambiguities, if any, were resolved by repeated sequencing (at least three replicates) from both ends. All the sequences were analyzed using different programs of Bioedit software, leading to identification of several SNPs and indels. A total of 15 sequence variations have been found in the present study with a frequency of 1 SNP per 87 bp. Haplotyping and intensive phenotyping of the selected inbred lines is in progress for identification of favorable allelic variants for carotenoid biosynthesis in maize.

Genetic Resources Program|Global Maize Program

Text in English

INT1617|INT3057

Click on an image to view it in the image viewer

Local cover image

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org