Peer review

Immature opaque 2 (o2) maize grains were compared with mature grains of o2 and normal maizes to determine the distribution and amino acid composition of protein fractions isolated by selective extraction. All the fractions are accumulated in o2 grains on development, except the albumins whose concentration decreases in the last stage of maturation. Each fraction has a nearly constant amino acid composition which is similar to that of the corresponding fractions present in normal grains. The data confirm that the main effect of the o2 gene is to alter the distribution of protein fractions by decreasing the proportion of accumulated zein and by increasing the proportions of salt-soluble proteins and G3-glutelins.

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