Normal view MARC view ISBD view

Coat protein sequence of an egyptian BYDV-PAV isolate

By: Waziri, H.M | Proceedimgs of an International Symposium Texcoco, Mex. (Mexico) 1-5 Sep 2002.
Contributor(s): Allam, E.K [coaut.] | El Din, A.S.G [coaut.] | El Gaffar, M.A [coaut.] | Henry, M.|McNab, A [eds.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Mexico, DF (Mexico) CIMMYT : 2002Description: p. 97-99.ISBN: 970-648-095-1.Subject(s): Aphids | Barley yellow dwarf virus | Chemical composition | DNA | Isolation techniques | Protein content | Rhopalosiphum padi | SeedlingsDDC classification: 632.3 Summary: BYDV-PAV is the most common strain in Egypt. Immunocapture IC/RT-PCR procedure was used to amplify fragments specific for BYDV-PA v: The system was further validated by sequencing fragments amplified. The cDNA of CP was inserted into plasmid to construct expression plasmid. The recombinant plasmid was identified by using restriction enzymes and sequenced to confirm PAV-CP gene in plasmid. The sequence of the coat protein of the PAV strain was identified and its amino acid sequence was deduced. Sequence comparison of the CP coding region between the PAV strains of BYDV have been done, and revealed the highest identity with the Moroccan strain (99%).Collection: CIMMYT Publications Collection
Tags from this library: No tags from this library for this title. Log in to add tags.
    average rating: 0.0 (0 votes)

BYDV-PAV is the most common strain in Egypt. Immunocapture IC/RT-PCR procedure was used to amplify fragments specific for BYDV-PA v: The system was further validated by sequencing fragments amplified. The cDNA of CP was inserted into plasmid to construct expression plasmid. The recombinant plasmid was identified by using restriction enzymes and sequenced to confirm PAV-CP gene in plasmid. The sequence of the coat protein of the PAV strain was identified and its amino acid sequence was deduced. Sequence comparison of the CP coding region between the PAV strains of BYDV have been done, and revealed the highest identity with the Moroccan strain (99%).

English

0208|AGRIS 0401|AL-Wheat Program|R02CIMPU

Juan Carlos Mendieta

CIMMYT Publications Collection

There are no comments for this item.

Log in to your account to post a comment.
baner

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Monday –Friday 9:00 am. 17:00 pm. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Lunes –Viernes 9:00 am. 17:00 pm. Si tiene cualquier pregunta, contáctenos a CIMMYT-Knowledge-Center@cgiar.org