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Coat protein sequence of an egyptian BYDV-PAV isolate

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Material type: Text

TextPublication details: Mexico, DF (Mexico) CIMMYT : 2002Description: p. 97-99ISBN:

970-648-095-1

Subject(s):

DDC classification:

632.3 HEN

Summary: BYDV-PAV is the most common strain in Egypt. Immunocapture IC/RT-PCR procedure was used to amplify fragments specific for BYDV-PA v: The system was further validated by sequencing fragments amplified. The cDNA of CP was inserted into plasmid to construct expression plasmid. The recombinant plasmid was identified by using restriction enzymes and sequenced to confirm PAV-CP gene in plasmid. The sequence of the coat protein of the PAV strain was identified and its amino acid sequence was deduced. Sequence comparison of the CP coding region between the PAV strains of BYDV have been done, and revealed the highest identity with the Moroccan strain (99%).

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Title notes ( 5 )

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Book	CIMMYT Knowledge Center: John Woolston Library	CIMMYT Publications Collection	632.3 HEN (Browse shelf(Opens below))	1	Available		Y629708

Total holds: 0

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Previous	632.3 HEN A maop-based approach towards clonning the Yd2 resistance gene in barley	632.3 HEN Exploitation of detected BYDV resistance genes in barley breeding	632.3 HEN Breeding for BYDV tolerance in wheat as a basis for a multiple stress tolerance strategy	632.3 HEN Coat protein sequence of an egyptian BYDV-PAV isolate	632.3 HEN Diagnosis of barley yellow dwarf virus and cereal yellow dwarf virus isolates in infected plants and vectors by group PCR and ELISA	632.3 HEN Preparing monoclonal antibodies against BYDV using viruses purified from naturally infected plants	632.3 HEN Improvement of BYDV-PAV detection in single aphid by using real-time RT-PCR fluorescent method	Next

BYDV-PAV is the most common strain in Egypt. Immunocapture IC/RT-PCR procedure was used to amplify fragments specific for BYDV-PA v: The system was further validated by sequencing fragments amplified. The cDNA of CP was inserted into plasmid to construct expression plasmid. The recombinant plasmid was identified by using restriction enzymes and sequenced to confirm PAV-CP gene in plasmid. The sequence of the coat protein of the PAV strain was identified and its amino acid sequence was deduced. Sequence comparison of the CP coding region between the PAV strains of BYDV have been done, and revealed the highest identity with the Moroccan strain (99%).

English

0208|AGRIS 0401|AL-Wheat Program|R02CIMPU

Juan Carlos Mendieta

CIMMYT Publications Collection

Knowledge Center Catalog

Coat protein sequence of an egyptian BYDV-PAV isolate

Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: CIMMYT Publications Collection Close shelf browser (Hides shelf browser)

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org

Knowledge Center Catalog

Coat protein sequence of an egyptian BYDV-PAV isolate

Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: CIMMYT Publications Collection Close shelf browser (Hides shelf browser)

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org