Knowledge Center Catalog

Local cover image
Local cover image

Doubled haploids in tropical maize : I. Effects of inducers and source germplasm on in vivo haploid induction rates

By: Contributor(s): Material type: ArticleArticleLanguage: English Publication details: USA : CSSA : Wiley, 2011.ISSN:
  • 1435-0653 (Online)
  • 0011-183X
Subject(s): Online resources: In: Crop Science v. 51, no. 4, p. 1498-1506Summary: The adoption of the doubled haploid (DH) technology in tropical maize (Zea mays L.) breeding programs is lagging behind that of temperate programs due to a lack of tropical haploid inducers and reliable information on the performance of temperate inducers under nontemperate conditions. The objective of this study was to determine the in vivo haploid induction ability of three temperate inducers crossed to a diverse set of tropical maize source germplasm under tropical conditions. Three experiments were conducted employing inducers as male parents to pollinate 120 source germplasm in three environments in Mexico. Haploid induction rates (HIR) obtained under field conditions were determined with two different haploid identification systems. Highly significant genotypic differences were detected among inducers and source germplasm for HIR but no interactions were observed between the two factors. Mean HIR under tropical conditions were similar to those reported for evaluations under temperate conditions indicating that temperate inducers can be employed for initiation of DH breeding programs in the tropics. Misclassification of diploids as haploids resulted in inflated HIR, particularly in highly variable source germplasm such as landraces or when expression of the identification marker was weak. We conclude that induction of haploidy is not a limiting factor for DH line production in tropical maize, but there is a need for the development of well-adapted tropical inducers.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection CIS-6356 (Browse shelf(Opens below)) Available
Total holds: 0

Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0011-183X

Peer review

The adoption of the doubled haploid (DH) technology in tropical maize (Zea mays L.) breeding programs is lagging behind that of temperate programs due to a lack of tropical haploid inducers and reliable information on the performance of temperate inducers under nontemperate conditions. The objective of this study was to determine the in vivo haploid induction ability of three temperate inducers crossed to a diverse set of tropical maize source germplasm under tropical conditions. Three experiments were conducted employing inducers as male parents to pollinate 120 source germplasm in three environments in Mexico. Haploid induction rates (HIR) obtained under field conditions were determined with two different haploid identification systems. Highly significant genotypic differences were detected among inducers and source germplasm for HIR but no interactions were observed between the two factors. Mean HIR under tropical conditions were similar to those reported for evaluations under temperate conditions indicating that temperate inducers can be employed for initiation of DH breeding programs in the tropics. Misclassification of diploids as haploids resulted in inflated HIR, particularly in highly variable source germplasm such as landraces or when expression of the identification marker was weak. We conclude that induction of haploidy is not a limiting factor for DH line production in tropical maize, but there is a need for the development of well-adapted tropical inducers.

Research and Partnership Program

Text in English

CIMMYT Informa No. 1761|Crop Science Society of America (CSSA)

INT1888

Click on an image to view it in the image viewer

Local cover image

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org