Knowledge Center Catalog

Local cover image
Local cover image

Evaluation of a real-time quantitative PCR assay for an effective screening of genotypes with FHB resistance and low mycotoxin accumulation in wheat and barley

By: Contributor(s): Material type: ArticleArticleLanguage: English Publication details: 2008. Szeged (Hungary) : Cereal Research Non-Profit Company,ISSN:
  • 0133-3720
  • 1788-9170 (Online)
Subject(s): In: Cereal Research Communications v. 36, no. Suppl. B., p. 147-148Summary: To estimate fungal biomass in grains infected with Fusarium graminearum causing Fusarium Head Blight (FHB), a real-time PCR assay was applied for the quantification of the pathogen DNA based on the copy number of trichothecene synthesis gene (Tri5) sequence. Using 64 lines of wheat and barley in a field, we found significant correlations among the disease severity, deoxynivalenot (DON) accumulation and the pathogen DNA amount in the grains from each inoculated spike. The strong relationships were maintained in the susceptible population showing high DON accumulation but not the resistance population with low DON accumulation. These results indicate that the PCR assay could be useful discarding the germplasm very susceptible to FHB disease.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)

Peer-review

To estimate fungal biomass in grains infected with Fusarium graminearum causing Fusarium Head Blight (FHB), a real-time PCR assay was applied for the quantification of the pathogen DNA based on the copy number of trichothecene synthesis gene (Tri5) sequence. Using 64 lines of wheat and barley in a field, we found significant correlations among the disease severity, deoxynivalenot (DON) accumulation and the pathogen DNA amount in the grains from each inoculated spike. The strong relationships were maintained in the susceptible population showing high DON accumulation but not the resistance population with low DON accumulation. These results indicate that the PCR assay could be useful discarding the germplasm very susceptible to FHB disease.

Research and Partnership Program

Tex in English

INT1237

CIMMYT Staff Publications Collection

Click on an image to view it in the image viewer

Local cover image

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org