Evaluation of a real-time quantitative PCR assay for an effective screening of genotypes with FHB resistance and low mycotoxin accumulation in wheat and barley
Material type: ArticleLanguage: English Publication details: 2008. Szeged (Hungary) : Cereal Research Non-Profit Company,ISSN:- 0133-3720
- 1788-9170 (Online)
Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
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Article | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-5429 (Browse shelf(Opens below)) | 1 | Available | 635385 |
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Peer-review
To estimate fungal biomass in grains infected with Fusarium graminearum causing Fusarium Head Blight (FHB), a real-time PCR assay was applied for the quantification of the pathogen DNA based on the copy number of trichothecene synthesis gene (Tri5) sequence. Using 64 lines of wheat and barley in a field, we found significant correlations among the disease severity, deoxynivalenot (DON) accumulation and the pathogen DNA amount in the grains from each inoculated spike. The strong relationships were maintained in the susceptible population showing high DON accumulation but not the resistance population with low DON accumulation. These results indicate that the PCR assay could be useful discarding the germplasm very susceptible to FHB disease.
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INT1237
CIMMYT Staff Publications Collection