Evaluation of a real-time quantitative PCR assay for an effective screening of genotypes with FHB resistance and low mycotoxin accumulation in wheat and barley
Jiro Murakami
Evaluation of a real-time quantitative PCR assay for an effective screening of genotypes with FHB resistance and low mycotoxin accumulation in wheat and barley - Szeged (Hungary) : Cereal Research Non-Profit Company, 2008. - Computer File|Printed
Peer-review
To estimate fungal biomass in grains infected with Fusarium graminearum causing Fusarium Head Blight (FHB), a real-time PCR assay was applied for the quantification of the pathogen DNA based on the copy number of trichothecene synthesis gene (Tri5) sequence. Using 64 lines of wheat and barley in a field, we found significant correlations among the disease severity, deoxynivalenot (DON) accumulation and the pathogen DNA amount in the grains from each inoculated spike. The strong relationships were maintained in the susceptible population showing high DON accumulation but not the resistance population with low DON accumulation. These results indicate that the PCR assay could be useful discarding the germplasm very susceptible to FHB disease.
Tex in English
0133-3720 1788-9170 (Online)
Fusarium
Vomitoxin
Gibberella zeae
PCR
Evaluation of a real-time quantitative PCR assay for an effective screening of genotypes with FHB resistance and low mycotoxin accumulation in wheat and barley - Szeged (Hungary) : Cereal Research Non-Profit Company, 2008. - Computer File|Printed
Peer-review
To estimate fungal biomass in grains infected with Fusarium graminearum causing Fusarium Head Blight (FHB), a real-time PCR assay was applied for the quantification of the pathogen DNA based on the copy number of trichothecene synthesis gene (Tri5) sequence. Using 64 lines of wheat and barley in a field, we found significant correlations among the disease severity, deoxynivalenot (DON) accumulation and the pathogen DNA amount in the grains from each inoculated spike. The strong relationships were maintained in the susceptible population showing high DON accumulation but not the resistance population with low DON accumulation. These results indicate that the PCR assay could be useful discarding the germplasm very susceptible to FHB disease.
Tex in English
0133-3720 1788-9170 (Online)
Fusarium
Vomitoxin
Gibberella zeae
PCR