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Soluble peroxidase activity in maize endosperm associated with maize weevil resistance

By: Contributor(s): Material type: ArticleArticleLanguage: English Publication details: USA : CSSA : Wiley, 2007.ISSN:
  • 1435-0653 (Online)
Subject(s): Online resources: In: Crop Science v. 47, no. 3, p. 1125-1130634781Summary: Plant peroxidases (PODs) are involved in resistance to pathogens and insects. This study investigated the role of POD in maize (Zea mays L.) resistance to the maize weevil, Sitophilus zeamais (Motsch.). Insect bioassays were performed under controlled conditions to assess maize weevil resistance. Peroxidase activity was measured in the major grain tissues using guaiacol and peroxide. Signifi cant variation (P > 0.001) among genotypes was observed in both the insect bioassay traits and POD activity. Peroxidase was detected in the embryo, endosperm, and pedicel, but it was not detected in the pericarp. Signifi cant correlations were found between endosperm POD activity and maize weevil resistance (r = 0.89, P < 0.001). Histological staining confi rmed POD activity in the vascular cylinder of the embryo, while activity in the endosperm was restricted to the aleurone layer. This study shows that POD activity is correlated with maize weevil resistance and may be used as a potential biochemical marker.
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Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection CIS-5029 (Browse shelf(Opens below)) 1 Available 634781
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Peer review

Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0011-183X

Plant peroxidases (PODs) are involved in resistance to pathogens and insects. This study investigated the role of POD in maize (Zea mays L.) resistance to the maize weevil, Sitophilus zeamais (Motsch.). Insect bioassays were performed under controlled conditions to assess maize weevil resistance. Peroxidase activity was measured in the major grain tissues using guaiacol and peroxide. Signifi cant variation (P > 0.001) among genotypes was observed in both the insect bioassay traits and POD activity. Peroxidase was detected in the embryo, endosperm, and pedicel, but it was not detected in the pericarp. Signifi cant correlations were found between endosperm POD activity and maize weevil resistance (r = 0.89, P < 0.001). Histological staining confi rmed POD activity in the vascular cylinder of the embryo, while activity in the endosperm was restricted to the aleurone layer. This study shows that POD activity is correlated with maize weevil resistance and may be used as a potential biochemical marker.

Text in English

Crop Science Society of America (CSSA)

MX-UAM,I 2004 GARCÍA D rf

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