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Testing for genetic purity in petunia and cyclamen seed using random amplified polymorphic DNA Markers

By: Contributor(s): Material type: ArticleArticleLanguage: English Publication details: 1997. USA : American Society for Horticultural Science,ISSN:
  • 0018-5345
  • 2327-9834 (Online)
Subject(s): In: HortScience v. 32, no. 2, p. 246-247629988Summary: This study examined the use of random amplified polymorphic DNA (RAPD) markers as a means to identify cultivars of petunia (Petunia hybrida Vilm) seedlings and cyclamen (Cyclamen persicum MiIL.) seeds and to determine te genetic purity within cyclamen seeds. Bulked samples of six petunia and five cyclamen hybrid cultivars, respectively, produced consistent RAPD marker profiles. Evaluation of individual seeds from a single cyclamen hybrid produced polymorphic banding patterns that were attributed to genetic variability present in the female and male inbred parents. These results show that RAPD markers can be used to quickly assess the genetic purity of selected cultivars of these two flowers seed crops.
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Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library Reprints Collection CIS-4054 (Browse shelf(Opens below)) 1 Available 629988
Total holds: 0

Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0018-5345

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This study examined the use of random amplified polymorphic DNA (RAPD) markers as a means to identify cultivars of petunia (Petunia hybrida Vilm) seedlings and cyclamen (Cyclamen persicum MiIL.) seeds and to determine te genetic purity within cyclamen seeds. Bulked samples of six petunia and five cyclamen hybrid cultivars, respectively, produced consistent RAPD marker profiles. Evaluation of individual seeds from a single cyclamen hybrid produced polymorphic banding patterns that were attributed to genetic variability present in the female and male inbred parents. These results show that RAPD markers can be used to quickly assess the genetic purity of selected cultivars of these two flowers seed crops.

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