Knowledge Center Catalog

Testing for genetic purity in petunia and cyclamen seed using random amplified polymorphic DNA Markers

Jianhua, Z.

Testing for genetic purity in petunia and cyclamen seed using random amplified polymorphic DNA Markers - USA : American Society for Horticultural Science, 1997. - Printed

Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0018-5345 Free Access

This study examined the use of random amplified polymorphic DNA (RAPD) markers as a means to identify cultivars of petunia (Petunia hybrida Vilm) seedlings and cyclamen (Cyclamen persicum MiIL.) seeds and to determine te genetic purity within cyclamen seeds. Bulked samples of six petunia and five cyclamen hybrid cultivars, respectively, produced consistent RAPD marker profiles. Evaluation of individual seeds from a single cyclamen hybrid produced polymorphic banding patterns that were attributed to genetic variability present in the female and male inbred parents. These results show that RAPD markers can be used to quickly assess the genetic purity of selected cultivars of these two flowers seed crops.


Text in English

0018-5345 2327-9834 (Online)

https://doi.org/10.21273/HORTSCI.32.2.246


Crop management
DNA
Genetic markers
Hybridization
Petunia
Polymorphism
Quality
Seed characteristics
Seed testing
Agricultural research

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