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Transformation of filamentous fungi with plasmid DNA by electroporation

By:

Richey, M.G

Contributor(s):

Material type: Article

ArticlePublication details: 1989ISSN:

0031-949X

Subject(s): DDC classification:

90-024852

In: Phytopathology (USA). (Aug 1989). v. 79(8) p. 844-847Summary: We investigated the use of high-voltage electric pulse to transform Fusarium solani and Aspergillus nidulans with DNA. This method involved exposure of fungal protoplasts, in the presence of vector DNA, to a high-amplitude of exponential-decay electric pulse of short duration (up to 123 msec) delivered from a Gene Pulser apparatus. The effects of pulse amplitude and duration on transformation frequency and cell viability were evaluated. The optimal transformation is rapid, simple to perform, and avoids the use of toxic chemicals. It may also provide an alternative method of introducing foreign DNA into fungi that cannot be transformed by traditional methods

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Title notes ( 4 )

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode	Item holds
Article	CIMMYT Knowledge Center: John Woolston Library	AGRIS Collection	90-024852 (Browse shelf(Opens below))	Available

Total holds: 0

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Previous	90-024036 Techniques and scoring procedures for starch gel electrophoresis of enzymes from maize (Zea mays L.)	90-024045 Changes in the zein composition of protein bodies during maize endosperm development	90-024271 15N2 fixation by bacteria associated with maize roots at a low partial O2 pressure	90-024852 Transformation of filamentous fungi with plasmid DNA by electroporation	90-024867 Toxin of Pyrenophora tritici-repentis: host-specificity, significance in disease, and inheritance of host reaction	90-027718 Drainage design and management:	90-028898 Reviewing agricultural research systems	Next

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We investigated the use of high-voltage electric pulse to transform Fusarium solani and Aspergillus nidulans with DNA. This method involved exposure of fungal protoplasts, in the presence of vector DNA, to a high-amplitude of exponential-decay electric pulse of short duration (up to 123 msec) delivered from a Gene Pulser apparatus. The effects of pulse amplitude and duration on transformation frequency and cell viability were evaluated. The optimal transformation is rapid, simple to perform, and avoids the use of toxic chemicals. It may also provide an alternative method of introducing foreign DNA into fungi that cannot be transformed by traditional methods

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Transformation of filamentous fungi with plasmid DNA by electroporation

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International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org

Knowledge Center Catalog

Transformation of filamentous fungi with plasmid DNA by electroporation

Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: AGRIS Collection Close shelf browser (Hides shelf browser)

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org