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Richey, M.G.

Transformation of filamentous fungi with plasmid DNA by electroporation - 1989 - Printed

ill. references

We investigated the use of high-voltage electric pulse to transform Fusarium solani and Aspergillus nidulans with DNA. This method involved exposure of fungal protoplasts, in the presence of vector DNA, to a high-amplitude of exponential-decay electric pulse of short duration (up to 123 msec) delivered from a Gene Pulser apparatus. The effects of pulse amplitude and duration on transformation frequency and cell viability were evaluated. The optimal transformation is rapid, simple to perform, and avoids the use of toxic chemicals. It may also provide an alternative method of introducing foreign DNA into fungi that cannot be transformed by traditional methods


English

ISSN: 0031-949X

Subjects--Topical Terms:
Acids
Cell structure
Cytoplasm
Cytoplasmic organelles
Energy
Energy sources
Fungi
Moulds
Natural resources
Nucleic acids
Nucleic compounds
Organic acids
Organic compounds
Physics
Plant diseases
Plants
Protoplasm
Resources
Genetics

Dewey Class. No.: 90-024852