Isolation of an alpha-type gliadin gene from Triticum durum Desf. and genetic polymorphism at the Gli-2 loci
Material type: ArticleLanguage: En Publication details: 1992ISSN:- 0394-9257
- Isolamento di un gene gliadina di tipo alfa da Triticum durum Desf. e polimorfismo genetico nei loci Gli-2
- 93-095289
Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
---|---|---|---|---|---|---|---|---|---|
Article | CIMMYT Knowledge Center: John Woolston Library | AGRIS Collection | 93-095289 (Browse shelf(Opens below)) | 1 | Available | 93-095289 |
Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: AGRIS Collection Close shelf browser (Hides shelf browser)
1 table; 1 graph; 29 ref. Summary (En)
Peer-review: No - Open Access: No
Genomic DNAs from Aegilops spp., Triticum spp., and related species were amplified by polymerase chain reaction (PCR). The oligonucleotides used as primers were terminal sequDEes of the coding region of an alpha-type gliadin gene. The PCR amplification produced an uniform band in all analysed Triticum and Aegilops genotypes, except Ae. speltoides. The PCR products of durum wheat cultivar Lira and T. urartu were cloned. Southern analysis of nulli-tetrasomic lines, carried out with digoxigenin labeled probe, demonstrated that the PCR amplification products represent alpha-type gliadin sequDEes. N-terminal sequDEe comparison of the T.
English
COMOD
AGRIS Collection