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Isolation of an alpha-type gliadin gene from Triticum durum Desf. and genetic polymorphism at the Gli-2 loci

By: Contributor(s): Material type: ArticleArticleLanguage: En Publication details: 1992ISSN:
  • 0394-9257
Other title:
  • Isolamento di un gene gliadina di tipo alfa da Triticum durum Desf. e polimorfismo genetico nei loci Gli-2
Subject(s): DDC classification:
  • 93-095289
In: Journal of Genetics and Breeding v. 46, no. 1, p. 41-4893-095289Summary: Genomic DNAs from Aegilops spp., Triticum spp., and related species were amplified by polymerase chain reaction (PCR). The oligonucleotides used as primers were terminal sequDEes of the coding region of an alpha-type gliadin gene. The PCR amplification produced an uniform band in all analysed Triticum and Aegilops genotypes, except Ae. speltoides. The PCR products of durum wheat cultivar Lira and T. urartu were cloned. Southern analysis of nulli-tetrasomic lines, carried out with digoxigenin labeled probe, demonstrated that the PCR amplification products represent alpha-type gliadin sequDEes. N-terminal sequDEe comparison of the T.
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1 table; 1 graph; 29 ref. Summary (En)

Peer-review: No - Open Access: No

Genomic DNAs from Aegilops spp., Triticum spp., and related species were amplified by polymerase chain reaction (PCR). The oligonucleotides used as primers were terminal sequDEes of the coding region of an alpha-type gliadin gene. The PCR amplification produced an uniform band in all analysed Triticum and Aegilops genotypes, except Ae. speltoides. The PCR products of durum wheat cultivar Lira and T. urartu were cloned. Southern analysis of nulli-tetrasomic lines, carried out with digoxigenin labeled probe, demonstrated that the PCR amplification products represent alpha-type gliadin sequDEes. N-terminal sequDEe comparison of the T.

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