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Isolation of an alpha-type gliadin gene from Triticum durum Desf. and genetic polymorphism at the Gli-2 loci

By: D'Ovidio, R.
Contributor(s): Porceddu, E [coaut.] | Tanzarella, O.A [coaut.].
Material type: materialTypeLabelArticlePublisher: 1992ISSN: 0394-9257.Other title: Isolamento di un gene gliadina di tipo alfa da Triticum durum Desf. e polimorfismo genetico nei loci Gli-2.Subject(s): Acids | Cell structure | Chromosomes | Genetic engineering | Genetic variation | Gramineae | Mathematical and statistical methods | Nucleic acids | Nucleic compounds | Nucleus | Organic acids | Polymorphism | Proteins | Triticum | Plant breeding AGROVOCDDC classification: 93-095289 In: Journal of Genetics and Breeding v. 46, no. 1, p. 41-4893-095289Summary: Genomic DNAs from Aegilops spp., Triticum spp., and related species were amplified by polymerase chain reaction (PCR). The oligonucleotides used as primers were terminal sequDEes of the coding region of an alpha-type gliadin gene. The PCR amplification produced an uniform band in all analysed Triticum and Aegilops genotypes, except Ae. speltoides. The PCR products of durum wheat cultivar Lira and T. urartu were cloned. Southern analysis of nulli-tetrasomic lines, carried out with digoxigenin labeled probe, demonstrated that the PCR amplification products represent alpha-type gliadin sequDEes. N-terminal sequDEe comparison of the T.Collection: AGRIS Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

AGRIS Collection 93-095289 (Browse shelf) 1 Available 93-095289
Total holds: 0

1 table; 1 graph; 29 ref. Summary (En)

Peer-review: No - Open Access: No

Genomic DNAs from Aegilops spp., Triticum spp., and related species were amplified by polymerase chain reaction (PCR). The oligonucleotides used as primers were terminal sequDEes of the coding region of an alpha-type gliadin gene. The PCR amplification produced an uniform band in all analysed Triticum and Aegilops genotypes, except Ae. speltoides. The PCR products of durum wheat cultivar Lira and T. urartu were cloned. Southern analysis of nulli-tetrasomic lines, carried out with digoxigenin labeled probe, demonstrated that the PCR amplification products represent alpha-type gliadin sequDEes. N-terminal sequDEe comparison of the T.

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