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Rapid detection of maize DNA sequence variation

By: Shattuck Eidens, D.M.
Contributor(s): Bell, R.N [coaut.] | McWhorter, V.C [coaut.] | Mitchell, J.T [coaut.].
Material type: materialTypeLabelArticlePublisher: 1991ISSN: 1050-3862.Subject(s): Biochemistry | DNA | Genes | Genetic markers AGROVOC | Genetic variation | Loci AGROVOC | Nucleotides | Zea mays AGROVOC | Genotypes AGROVOCDDC classification: 93-074116 In: Gene analysis techniques and applications v. 8, no. 8, p. 240-24593-074116Summary: The allele-specific polymerase chain reaction (ASPCR) has been used to determine the genotype of maize lines at two loci, wx and NPI288. The ASPCR method uses allele-specific oligonucleotide primers in PCR amplifications to amplify and discriminate simultaneously between polymorphic alleles. The success of this technique relies on the specific failure of PCR to amplify, with primers that do not perfectly match the DNA sequence of one of the allelic variants. Amplification results were evaluated by dot-blot hybridization using an alkaline-phosphatase-coupled probe. The technique's speed, accuracy sensitivity and high throughput make itCollection: AGRIS Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

AGRIS Collection 93-074116 (Browse shelf) 1 Available 93-074116
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The allele-specific polymerase chain reaction (ASPCR) has been used to determine the genotype of maize lines at two loci, wx and NPI288. The ASPCR method uses allele-specific oligonucleotide primers in PCR amplifications to amplify and discriminate simultaneously between polymorphic alleles. The success of this technique relies on the specific failure of PCR to amplify, with primers that do not perfectly match the DNA sequence of one of the allelic variants. Amplification results were evaluated by dot-blot hybridization using an alkaline-phosphatase-coupled probe. The technique's speed, accuracy sensitivity and high throughput make it

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