MARC details
| 000 -LEADER |
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| fixed length control field |
01712nab a22004097a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G51218 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
121211b |||p||p||||||| |z||| | |
| 022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1050-3862 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
En |
| 043 ## - GEOGRAPHIC AREA CODE |
|---|
| Geographic area code |
US |
| 072 #0 - SUBJECT CATEGORY CODE |
|---|
| Subject category code |
F30 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
93-074116 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Shattuck Eidens, D.M. |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Rapid detection of maize DNA sequence variation |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Date of publication, distribution, etc. |
1991 |
| 340 ## - PHYSICAL MEDIUM |
|---|
| Material base and configuration |
Printed |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The allele-specific polymerase chain reaction (ASPCR) has been used to determine the genotype of maize lines at two loci, wx and NPI288. The ASPCR method uses allele-specific oligonucleotide primers in PCR amplifications to amplify and discriminate simultaneously between polymorphic alleles. The success of this technique relies on the specific failure of PCR to amplify, with primers that do not perfectly match the DNA sequence of one of the allelic variants. Amplification results were evaluated by dot-blot hybridization using an alkaline-phosphatase-coupled probe. The technique's speed, accuracy sensitivity and high throughput make it |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
English |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
US (DNAL QH442.G446) |
| 595 ## - COLLECTION |
|---|
| Collection |
AGRIS Collection |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Biochemistry |
| 9 (RLIN) |
1021 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
DNA |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genes |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1848 |
| Topical term or geographic name as entry element |
Genetic markers |
| Miscellaneous information |
AGROVOC |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genetic variation |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1129 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1172 |
| Topical term or geographic name as entry element |
Loci |
| Miscellaneous information |
AGROVOC |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Nucleotides |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1314 |
| Topical term or geographic name as entry element |
Zea mays |
| Miscellaneous information |
AGROVOC |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1134 |
| Topical term or geographic name as entry element |
Genotypes |
| Miscellaneous information |
AGROVOC |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Bell, R.N., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
McWhorter, V.C., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mitchell, J.T., |
| Relator term |
coaut. |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Gene analysis techniques and applications |
| Note |
93-074116 |
| Related parts |
v. 8, no. 8, p. 240-245 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |