Knowledge Center Catalog

Properties of nucleosides diphosphatases in purified membrane fractions from maize coleoptiles, 1: study of latency

By: Contributor(s): Material type: ArticleArticleLanguage: En Publication details: 1981Subject(s): DDC classification:
  • 81-663982
 In: Plant Science Letters v. 20, no. 4, p. 379-38681-663982Summary: Evidence is given for the presence of nucleoside diphosphatase (NDPase) activities in two membrane fractions from maize coleoptiles, one enriched in endoplasmic reticulum (ER) and the other in plasma membranes (PM). The NDPase activity associated with the ER-rich fraction appears only after treatment of membranes with detergent. Enzyme inactivation experiments using trypsin were performed on intact and Triton X-100-treated vesicles. They suggest that in the endoplasmic reticulum-rich fraction, most of enzymatic sites have a lumenal location and that in plasma membrane vesicles, only 40-50 per cent of enzymatic sites are not freely accessible to nucleosides
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library AGRIS Collection 81-663982 (Browse shelf(Opens below)) 1 Available 81-663982
Total holds: 0

18 ref.; Summary (En)

Evidence is given for the presence of nucleoside diphosphatase (NDPase) activities in two membrane fractions from maize coleoptiles, one enriched in endoplasmic reticulum (ER) and the other in plasma membranes (PM). The NDPase activity associated with the ER-rich fraction appears only after treatment of membranes with detergent. Enzyme inactivation experiments using trypsin were performed on intact and Triton X-100-treated vesicles. They suggest that in the endoplasmic reticulum-rich fraction, most of enzymatic sites have a lumenal location and that in plasma membrane vesicles, only 40-50 per cent of enzymatic sites are not freely accessible to nucleosides

English

COMOD

AGRIS Collection


International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org