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On the sequential determination of alfa-amylase transport and cell wall breakdown in germinating seeds of hordeum vulgare [barley, immunofluorescence, Calcofluor, gibberellic acid, "abrasion", beta-glucan breakdown, malting]

By: Material type: ArticleArticleLanguage: En Publication details: 1980ISSN:
  • 0105-1938
Subject(s): DDC classification:
  • 81-644367
In: Carlsberg Research Communications v. 45, no. 3, p. 177-18481-644367Summary: By means of immunohistochemical alpha-amylase determination techniques and Calcofluor white M2R New staining of residual endosperm cell walls it was possible to observe transport of alpha-amylase and breakdown of cell wall material in germinating seeds of Hordeum vulgare cv. Nordal. When barley seeds of which the distal tip had been removed, were treated with gibberellic acid, the importance of the distal aleurone cells in the production of both alpha-amylase and cell wall breakdown hydrolases was clearly seen
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Article CIMMYT Knowledge Center: John Woolston Library AGRIS Collection 81-644367 (Browse shelf(Opens below)) 1 Available 81-644367
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10 ref. Summary (En)

By means of immunohistochemical alpha-amylase determination techniques and Calcofluor white M2R New staining of residual endosperm cell walls it was possible to observe transport of alpha-amylase and breakdown of cell wall material in germinating seeds of Hordeum vulgare cv. Nordal. When barley seeds of which the distal tip had been removed, were treated with gibberellic acid, the importance of the distal aleurone cells in the production of both alpha-amylase and cell wall breakdown hydrolases was clearly seen

English

Carlsberg Lab., Copenhagen (Denmark)|COMOD

AGRIS Collection


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