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Regeneration of plants from mesocotyl tissue cultures of immature embryos of Zea mays L

By: Contributor(s): Material type: ArticleArticleLanguage: En Publication details: 1980Subject(s): DDC classification:
  • 80-549503
 In: Plant Science Letters v. 17, no. 3, p. 339-34480-549503Summary: Callus cultures have been established from mesocotyl tissues taken from immature embryos of one experimental hybrid homozygous fl-2. They were initiated and maintained on a modified Murashige and Skoog medium. Regeneration of complete plants from these calluses was accomplished by a subculture of the callus on a medium containing 0.25 mg 2,4-D and NAA for 40 days. Afterwards they were transformed to a medium containing NAA and 2-i-P free of 2,4-D
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Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library AGRIS Collection 80-549503 (Browse shelf(Opens below)) 1 Available 80-549503
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9 ref.; Summary (En) included on tape

Callus cultures have been established from mesocotyl tissues taken from immature embryos of one experimental hybrid homozygous fl-2. They were initiated and maintained on a modified Murashige and Skoog medium. Regeneration of complete plants from these calluses was accomplished by a subculture of the callus on a medium containing 0.25 mg 2,4-D and NAA for 40 days. Afterwards they were transformed to a medium containing NAA and 2-i-P free of 2,4-D

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