Plant regeneration in vitro from embryogenic cultures of spring- and winter-type barley (Hordeum vulgare L.) varieties
Material type:
ArticleLanguage: En Publication details: 1987ISSN: - 1432-2242 (Revista en electrónico)
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Article | CIMMYT Knowledge Center: John Woolston Library | Reprints Collection | REP-4232 (Browse shelf(Opens below)) | 1 | Available | 617561 |
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Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
Immature embryos of 41 lines were screened in vitro for callus induction andsomatic embryogenesis on different media. The use of modified Murashige & Skoog and CC media, both supplemented with 1 g casein hydrolysate/litre and containingagarose instead of agar, gave the highest frequencies of somatic embryo induction. Plants were regenerated from 23 of the lines. There were strong genotypic effects on the capacity and frequency of embryogenic callus formation;cv. Golden Promise gave the best results. Experiments with field-grown material over 3 years showed that environmental factors also strongly influenced somatic embryogenesis and plant
English
Springer
Reprints Collection
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