Knowledge Center Catalog

Plant regeneration in vitro from embryogenic cultures of spring- and winter-type barley (Hordeum vulgare L.) varieties

Luhrs, R.

Plant regeneration in vitro from embryogenic cultures of spring- and winter-type barley (Hordeum vulgare L.) varieties - 1987 - Printed

Illustrations, tables, refernces 24-25 Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752

Immature embryos of 41 lines were screened in vitro for callus induction andsomatic embryogenesis on different media. The use of modified Murashige & Skoog and CC media, both supplemented with 1 g casein hydrolysate/litre and containingagarose instead of agar, gave the highest frequencies of somatic embryo induction. Plants were regenerated from 23 of the lines. There were strong genotypic effects on the capacity and frequency of embryogenic callus formation;cv. Golden Promise gave the best results. Experiments with field-grown material over 3 years showed that environmental factors also strongly influenced somatic embryogenesis and plant


English

1432-2242 (Revista en electrónico)


Barley
Callus
Embryonic development
Germination
Hordeum vulgare
Tissue culture
Vegetative propagation
Genotypes

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