Differential expression of a gene for a methionine-rich storage protein in maize
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ArticleLanguage: En Publication details: 1988Subject(s):
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Molecular and General Genetics v. 211, no. 3, p. 477-484615038Summary: On the basis of the sequence of the N-terminal 30 amino acids of a 10 kD methionine-rich zein protein, 2 mixed oligonucleotides were synthesized and usedto probe a maize endosperm cDNA library. A full-length cDNA clone encoding the 10 kD zein was isolated;its nucleotide sequence predicted a polypeptide of 129 amino acids, preceded by a signal peptide of 21 amino acids. The predicted polypeptide was unique in its extremely high content of methionine (22.5%). The inbred line BSSS53, which has increased seed methionine, was compared to W23, a standard inbred line. Northern blot analysis showed that the relative RNA levelsfor the 10 kD zein were enhanced
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| Article | CIMMYT Knowledge Center: John Woolston Library | Reprints Collection | REP-4391 (Browse shelf(Opens below)) | 1 | Available | 615038 |
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Illustrations, tables, references p. 483-484
On the basis of the sequence of the N-terminal 30 amino acids of a 10 kD methionine-rich zein protein, 2 mixed oligonucleotides were synthesized and usedto probe a maize endosperm cDNA library. A full-length cDNA clone encoding the 10 kD zein was isolated;its nucleotide sequence predicted a polypeptide of 129 amino acids, preceded by a signal peptide of 21 amino acids. The predicted polypeptide was unique in its extremely high content of methionine (22.5%). The inbred line BSSS53, which has increased seed methionine, was compared to W23, a standard inbred line. Northern blot analysis showed that the relative RNA levelsfor the 10 kD zein were enhanced
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