Differential expression of a gene for a methionine-rich storage protein in maize
Kirihara, J.A.
Differential expression of a gene for a methionine-rich storage protein in maize - 1988 - Printed
Illustrations, tables, references p. 483-484
On the basis of the sequence of the N-terminal 30 amino acids of a 10 kD methionine-rich zein protein, 2 mixed oligonucleotides were synthesized and usedto probe a maize endosperm cDNA library. A full-length cDNA clone encoding the 10 kD zein was isolated;its nucleotide sequence predicted a polypeptide of 129 amino acids, preceded by a signal peptide of 21 amino acids. The predicted polypeptide was unique in its extremely high content of methionine (22.5%). The inbred line BSSS53, which has increased seed methionine, was compared to W23, a standard inbred line. Northern blot analysis showed that the relative RNA levelsfor the 10 kD zein were enhanced
English
Methionine
Prolamines
Seed
Seeds
Zea mays
Zea mays
Differential expression of a gene for a methionine-rich storage protein in maize - 1988 - Printed
Illustrations, tables, references p. 483-484
On the basis of the sequence of the N-terminal 30 amino acids of a 10 kD methionine-rich zein protein, 2 mixed oligonucleotides were synthesized and usedto probe a maize endosperm cDNA library. A full-length cDNA clone encoding the 10 kD zein was isolated;its nucleotide sequence predicted a polypeptide of 129 amino acids, preceded by a signal peptide of 21 amino acids. The predicted polypeptide was unique in its extremely high content of methionine (22.5%). The inbred line BSSS53, which has increased seed methionine, was compared to W23, a standard inbred line. Northern blot analysis showed that the relative RNA levelsfor the 10 kD zein were enhanced
English
Methionine
Prolamines
Seed
Seeds
Zea mays
Zea mays