DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat
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Biochemical Genetics v. 24, no. 7-8, p. 579-596615053Summary: EcoRI, BamHI and HindIII restriction digests of DNA from nullisomic- tetrasomic and intervarietal chromosome substitution lines were probed separately with a HMW glutenin cDNA. Five cultivars were represented. The hybridizing fragments appeared to contain single gene copies. Restriction fragment polymorphism associated with previously established allelic differencesbetween varieties was observed. Electrophoretic mobility of a HMW subunit and the length of the central repetitive section of the gene encoding it were related.
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| Article | CIMMYT Knowledge Center: John Woolston Library | Reprints Collection | REP-3800 (Browse shelf(Opens below)) | 1 | Available | 615053 |
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EcoRI, BamHI and HindIII restriction digests of DNA from nullisomic- tetrasomic and intervarietal chromosome substitution lines were probed separately with a HMW glutenin cDNA. Five cultivars were represented. The hybridizing fragments appeared to contain single gene copies. Restriction fragment polymorphism associated with previously established allelic differencesbetween varieties was observed. Electrophoretic mobility of a HMW subunit and the length of the central repetitive section of the gene encoding it were related.
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