Purification and serology of a Japanese isolate of barley yellow dwarf virus
Material type: ArticleLanguage: En Publication details: 1985Subject(s): In: Annals of the Phytopathological Society of Japan v. 51, no. 2, p. 152-158615284Summary: BYDV was purified from infected barley plants by an improved procedure whichyielded 44 æg virus/100 g tissues. The isolate had isometric particles, diam. 27.3 nm and a UV absorbance spectrum with an av. A260/A280 ratio of 1.71. In ELISA using the double antibody sandwich method, the virus reacted with homologous antiserum but not with antiserum to potato leafroll virus and vice versa. It reacted slightly with heterologous antisera to other luteoviruses suchas PLRV, soyabean dwarf and beet western yellows viruses in agar gel double diffusion tests.Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
---|---|---|---|---|---|---|---|---|---|
Article | CIMMYT Knowledge Center: John Woolston Library | Reprints Collection | REP-3671 (Browse shelf(Opens below)) | 1 | Available | 615284 |
Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: Reprints Collection Close shelf browser (Hides shelf browser)
Illustrations, tables, literature cited p. 157
BYDV was purified from infected barley plants by an improved procedure whichyielded 44 æg virus/100 g tissues. The isolate had isometric particles, diam. 27.3 nm and a UV absorbance spectrum with an av. A260/A280 ratio of 1.71. In ELISA using the double antibody sandwich method, the virus reacted with homologous antiserum but not with antiserum to potato leafroll virus and vice versa. It reacted slightly with heterologous antisera to other luteoviruses suchas PLRV, soyabean dwarf and beet western yellows viruses in agar gel double diffusion tests.
English
Reprints Collection