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Polymorphism at the hor 1 locus of barley (Hordeum vulgare L.)

By: Shewry, P.R.
Contributor(s): Bunce, N.A.C [coaut.] | Forde, B.G [coaut.] | Kreis, M [coaut.].
Material type: materialTypeLabelArticlePublisher: 1985Subject(s): Barley | DNA | Enzymes | Genetic maps | Genomes | Peptides | Polymorphism | Prolamines | Proteins In: Biochemical Genetics v. 23, no. 5-6, p. 391-404609965Summary: Two-dimensional electrophoretic analysis of C-hordein fractions from 6 cultivars with different alleles at the Hor1 locus (Hor1Pr, Ar, Ke, Ju, Ho and So) showed extensive polymorphism. A total of 34 major polypeptides were identified, with 4-18 present in each cultivar. For each cultivar, 6-10 restriction fragments of genomic DNA hybridized to a cDNA clone related to C- hordein;the total number of restriction fragments was 22, and most pairs of cultivars had more restriction fragments than polypeptides in common. There wereestimated to be 20-30 C-hordein genes per haploid genome. It is concluded that cultivars differ mainly in the extent of gene andCollection: Reprints Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

Reprints Collection REP-3661 (Browse shelf) 1 Available 609965
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Illustrations, tables, references p. 403-404

Two-dimensional electrophoretic analysis of C-hordein fractions from 6 cultivars with different alleles at the Hor1 locus (Hor1Pr, Ar, Ke, Ju, Ho and So) showed extensive polymorphism. A total of 34 major polypeptides were identified, with 4-18 present in each cultivar. For each cultivar, 6-10 restriction fragments of genomic DNA hybridized to a cDNA clone related to C- hordein;the total number of restriction fragments was 22, and most pairs of cultivars had more restriction fragments than polypeptides in common. There wereestimated to be 20-30 C-hordein genes per haploid genome. It is concluded that cultivars differ mainly in the extent of gene and

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