Polymorphism at the hor 1 locus of barley (Hordeum vulgare L.)
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ArticleLanguage: En Publication details: 1985Subject(s):
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Biochemical Genetics v. 23, no. 5-6, p. 391-404609965Summary: Two-dimensional electrophoretic analysis of C-hordein fractions from 6 cultivars with different alleles at the Hor1 locus (Hor1Pr, Ar, Ke, Ju, Ho and So) showed extensive polymorphism. A total of 34 major polypeptides were identified, with 4-18 present in each cultivar. For each cultivar, 6-10 restriction fragments of genomic DNA hybridized to a cDNA clone related to C- hordein;the total number of restriction fragments was 22, and most pairs of cultivars had more restriction fragments than polypeptides in common. There wereestimated to be 20-30 C-hordein genes per haploid genome. It is concluded that cultivars differ mainly in the extent of gene and
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Article | CIMMYT Knowledge Center: John Woolston Library | Reprints Collection | REP-3661 (Browse shelf(Opens below)) | 1 | Available | 609965 |
Illustrations, tables, references p. 403-404
Two-dimensional electrophoretic analysis of C-hordein fractions from 6 cultivars with different alleles at the Hor1 locus (Hor1Pr, Ar, Ke, Ju, Ho and So) showed extensive polymorphism. A total of 34 major polypeptides were identified, with 4-18 present in each cultivar. For each cultivar, 6-10 restriction fragments of genomic DNA hybridized to a cDNA clone related to C- hordein;the total number of restriction fragments was 22, and most pairs of cultivars had more restriction fragments than polypeptides in common. There wereestimated to be 20-30 C-hordein genes per haploid genome. It is concluded that cultivars differ mainly in the extent of gene and
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