Isoenzimas como marcadores geneticos : Na identificacao de nove linhagens de milho
Material type:
ArticleLanguage: English Publication details: 1982. Brasilia (Brazil) : EMBRAPA,ISSN: - 0100-204X
- 1678-3921 (Online)
- Isoenzimes as genetic markers to identify nine corn lines
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Article | CIMMYT Knowledge Center: John Woolston Library | Reprints Collection | REP-2990 (Browse shelf(Opens below)) | 1 | Available | 601610 |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0100-204X
Nove linhagens de milho são identificadas empregando-se locos predeterminados através de padrões enzimáticos. As isoenzimas, produtos primários de ação gênica, são visualizadas na técnica da eletroforese, com substratos e corantes especiais. Os sistemas utilizados são álcool desidrogenase, catalase, peroxidase, esterase e fosfatase ácida. A presença deste ou daquele alelo em determinado loco corresponde à banda observada, permitindo, desta forma, o conhecimento do genótipo da linhagem. Apenas quatro locos em esterase diferenciaram as nove linhagens.
Nine corn lines are identified through enzymatic pattemns and based on loci very weII determnined. Isoenzymes, primary products of gene action, are observed using electrophoresis technic with special substrates and dye. The systems used are alcohol dehidrogenase, catalase, peroxidase, esterase and acid phosphatase. The presence of the aliele in special locus corresponds lo lhe band observed, and by this way the genotype is known. OnIy four loci in esterase d,fferenced the nine lines.
Text in Portuguese, title and abstract available in English
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