MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03571nab|a22003617a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
69046 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20251014115505.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
20251s2025|||||-us||p|op||||00||0|eng|dd |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1932-6203 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1371/journal.pone.0325548 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Gillman, L. |
| 9 (RLIN) |
39651 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Optimizing genomic diversity assessments for conservation of Bromus auleticus (Trinius ex Nees) using individual and pooled sequencing |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
San Francisco, CA (United States of America) : |
| Name of publisher, distributor, etc. |
Public Library of Science, |
| Date of publication, distribution, etc. |
2025. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Bromus auleticus, a valuable forage grass native to the Pampa biome, is currently undergoing genetic erosion. Therefore, it is essential to assess appropriate methodologies for developing population genomic studies that will contribute to the conservation of this genetic resource. In this study, we evaluated five accessions using two genotyping strategies: individual sequencing (ind-seq) and pooled sequencing (pool-seq). To assess methodologies effectiveness, the correlation between allele frequencies calculated using each approach was investigated, as well as genetic diversity and population structure. These comparisons explicitly accounted for the potential effects of factors such as sample size, missing data, sequencing depth, and minor allele frequencies. The highest values of frequencies concordance and percentage of SNPs in common between ind-seq and pool-seq were achieved using a sample size of 30-60 plants per accession. These values were obtained with a maximum missing data threshold of 10% and a less strict minimum allele frequency threshold for pool-seq (0.01) compared to ind-seq (0.05). Pool-seq required a higher sequencing depth per accession (4.8 million reads) compared to ind-seq (0.9 million reads) to achieve similar allele frequencies. Pools of 50 individuals yielded the highest number of polymorphic sites, averaging over 9,000 per accession at a sequencing depth of 4.8 Mr. Under these conditions, pool-seq consistently resulted in an average of 0.09 higher expected heterozygosity and a 0.24 lower allelic richness compared to ind-seq in all accessions. Population structure inferred with both methodologies confirmed the outcrossing nature of B. auleticus and aligned with the geographical origin of each accession. The average inbreeding coefficient of 0.2 evidence inbreeding, which highlights the importance of conservation efforts for this valuable plant genetic resource. Based on these findings, we propose two workflows for conducting population genomics studies on Bromus auleticus. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 597 ## - CGIAR Initiative |
|---|
| Donor or Funder |
Agencia Nacional de Investigación e Innovación (ANII) |
| -- |
Instituto Nacional de Investigación Agropecuaria (INIA) |
| -- |
Centro Universitario Regional del Este (CURE) |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genetic diversity (resource) |
| Miscellaneous information |
AGROVOC |
| 9 (RLIN) |
2974 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genotyping |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
22057 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Sequence analysis |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
39652 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Single nucleotide polymorphisms |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
10805 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Condón, F. |
| 9 (RLIN) |
39653 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Petroli, C.D. |
| Miscellaneous information |
Genetic Resources Program |
| Field link and sequence number |
CPEC01 |
| 9 (RLIN) |
1408 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Rivas, M. |
| 9 (RLIN) |
39654 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
PLOS One |
| Related parts |
v. 20, no. 6, art. e0325548 |
| Place, publisher, and date of publication |
San Francisco, CA (United States of America) : Public Library of Science, 2025. |
| Record control number |
G94957 |
| International Standard Serial Number |
1932-6203 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/35801 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |