MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03232nab|a22003977a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
61625 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20231016185004.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
200331s2020||||sz |||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
2223-7747 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.3390/plants9040459 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Chaikam, V. |
| 9 (RLIN) |
936 |
| Field link and sequence number |
INT3356 |
| Miscellaneous information |
Global Maize Program |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Improving the efficiency of colchicine-based chromosomal doubling of maize haploids |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Basel (Switzerland) : |
| Name of publisher, distributor, etc. |
MDPI, |
| Date of publication, distribution, etc. |
2020. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Production and use of doubled haploids (DH) is becoming an essential part of maize breeding programs worldwide as DH lines offer several advantages in line development and evaluation. One of the critical steps in maize DH line production is doubling the chromosomes of in vivo-derived haploids so that naturally sterile haploids become reproductively fertile diploids (DH) to produce seed. This step of artificially doubling the chromosomes is labor-intensive and costly; hence, optimizing protocols to improve the doubling success is critical for achieving efficiencies in the DH production pipelines. Immersion of 3–4-day old germinating haploid seedlings in colchicine solution is commonly used for chromosome doubling in large-scale maize DH line production. This manuscript presents a new method of colchicine application to haploid seedlings that showed superior doubling rates compared to other methods like standard seedling immersion, seed immersion, root immersion, and direct application of colchicine solution to the seedlings at V2 stage in the greenhouse trays. The new method involves immersing the crown region of the haploid seedlings along with all the seedling roots at V2 stage in the colchicine solution. Further experiments to optimize this method indicated that increasing colchicine concentration had a very positive effect on overall success rate in chromosomal doubling, while not drastically affecting survival rate. The optimized method showed on average 5.6 times higher overall success rate (OSR) compared to the standard haploid seedling immersion method which was the second-best method in our experiments. This improved method of colchicine application saves resources by reducing the number of haploids to be generated and handled in a maize DH production pipeline. |
| 526 ## - STUDY PROGRAM INFORMATION NOTE |
|---|
| Program name |
Maize CRP |
| Maize Flagship Projects |
FP2 - Novel tools, technologies and traits for improving genetic gains and breeding efficiency |
| -- |
FP3 - Stress resilient and nutritious maize |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Martinez, L. : Not in IRS Staff list but CIMMYT Affiliation |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Ochieng, J.A.W. : Not in IRS Staff list but CIMMYT Affiliation |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Maize |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1925 |
| Topical term or geographic name as entry element |
Haploidy |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
7014 |
| Topical term or geographic name as entry element |
Chromosomes |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
12122 |
| Topical term or geographic name as entry element |
Colchicine |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Gowda, M. |
| 9 (RLIN) |
795 |
| Field link and sequence number |
I1705963 |
| Miscellaneous information |
Global Maize Program |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Martinez, L. |
| 9 (RLIN) |
3372 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ochieng, J. |
| 9 (RLIN) |
31316 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hamilton Amoshe Omar |
| 9 (RLIN) |
12123 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Prasanna, B.M. |
| Miscellaneous information |
Global Maize Program |
| Field link and sequence number |
INT3057 |
| 9 (RLIN) |
887 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Plants |
| Related parts |
v. 9, no. 4, art. 459 |
| Place, publisher, and date of publication |
Basel (Switzerland) : MDPI, 2020. |
| International Standard Serial Number |
2223-7747 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/20818 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |