Knowledge Center Catalog

Cloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species (Record no. 28280)

MARC details
000 -LEADER
fixed length control field 03194nab a22003737a 4500
001 - CONTROL NUMBER
control field G94538
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20230731154935.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210706s2010 cc |||p|op||| 00| 0 chi d
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0253-9772
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.3724/SP.J.1005.2010.00613
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title chi
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-6113
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Lin-hai Wang
9 (RLIN) 20836
245 10 - TITLE STATEMENT
Title Cloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Beijing (China) :
Name of publisher, distributor, etc. Science Press,
Date of publication, distribution, etc. 2010.
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0253-9772
520 ## - SUMMARY, ETC.
Summary, etc. The common wheat relative species are important germplasm for wheat breeding. In the present study, novel allelic variants at Glu-B3 locus were cloned to provide gene resources for wheat quality improvement. Four Glu-B3-locus specific primer sets LB1F/LB1R, LB2F/LB2R, LB3F/LB3R, and LB4F/LB4R were employed to isolate novel allelic variants of GluB3-1, GluB3-2, GluB3-3, and GluB3-4 from seven common wheat relative species, i.e., T. durum, T. dicoccum, T. dicoccoides, Aegilops longissima, Ae. searsii, Ae. Bicornis, and Ae. speltoides, and the software MEGA 4 was used to construct a phylogenetic tree. In total, 16 novel allelic variants of GluB3-1, GluB3-3, and GluB3-4 genes were isolated from the seven common wheat relative species, designated GluB3-16, GluB3-35, GluB3-36, GluB3-37, GluB3-46, GluB3-47, GluB3-48, GluB3-49, GluB3-410, GluB3-411, GluB3-412, GluB3-413, GluB3-414, GluB3-415, GluB3-416 and GluB3-417, respectively. In detail, GluB3-16 was cloned from T. dicoccoides with LB1F/LB1R, and the molecular weight of the deduced amino acid was 39.2 kDa. GluB3-35, GluB3-36, and GluB3-37 were isolated from T. durum and T. dicoccum with the primer set LB3F/LB3R, and the molecular weights of their deduced peptides were 44.5 kDa (GluB3-36) and 44.6 kDa (GluB3-35 and GluB3-37). The molecular weight of deduced peptides of GluB3-4 ranged from 38.6 kDa (GluB3-414) to 42.5 kDa (GluB3-413). All the 16 new allelic variants showed a single open reading frame (ORF), and their deduced amino-acid sequences had a typical sequence structure of LMW-GS. The allelic variants at Glu-B3 locus identified in common wheat relative species provide potential gene resources for wheat quality breeding and gene transformation. The results suggested that these Glu-B3 genes originated from different evolution processes.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note Text in Chinese
594 ## - STAFFID
StaffID INT2411
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Triticum aestivum
Source of heading or term AGROVOC
9 (RLIN) 1296
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Glutenins
Source of heading or term AGROVOC
9 (RLIN) 1137
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Phylogenetics
Source of heading or term AGROVOC
9 (RLIN) 30107
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Zhou Min
9 (RLIN) 20928
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Li Hui-Ling
9 (RLIN) 20929
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name He Zhonghu
Miscellaneous information Global Wheat Program
Field link and sequence number INT2411
9 (RLIN) 838
700 0# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 377
Personal name Xianchun Xia
773 0# - HOST ITEM ENTRY
Title Hereditas (Beijing)
Related parts v. 32, no. 6, p. 613-624
Place, publisher, and date of publication Beijing (China) : Science Press, 2010.
International Standard Serial Number 0253-9772
856 4# - ELECTRONIC LOCATION AND ACCESS
Link text Access only for CIMMYT Staff
Uniform Resource Identifier https://hdl.handle.net/20.500.12665/704
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme Dewey Decimal Classification
Suppress in OPAC No
Holdings
Date last seen Total Checkouts Full call number Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/05/2017   CIS-6113 07/05/2017 Article Not Lost Dewey Decimal Classification     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/05/2017

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org