000			03088nam a22004217a 4500
001			G94887
003			MX-TxCIM
005			20211006082355.0
008			121211s ||||f| 0 p|p||0|| |
040			_aMX-TxCIM
090			_aCIS-6278
100	1		_aLi Liu _uInternational Triticeae Mapping Initiative 20; Wheat Genomics in China 2; Beijing (China); 1-5 Sep 2010
245	0	0	_aComparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat
260			_aBeijing (China) _bChinese Academy of Agricultural Sciences : _c2010
300			_ap. 196
500			_aAbstract only
520			_aAt the Glu-A3 locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles Glu-A3e and Glu-A3d could not be routinely distinguished from Glu-A3f and Glu-A3g, respectively, based on SDS-PAGE, and the allele Glu-A3a could not be differentiated from Glu-A3c by MALDI-TOF-MS. At the Glu-B3 locus, alleles Glu-B3a, Glu-B3b, Glu-B3c,Glu-B3g, Glu-B3h and Glu-B3j could be clearly identified by all four methods, whereas Glu-B3ab, Glu-B3ac, Glu-B3ad could only be identified by the 2-DE method. At the Glu-D3 locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the Glu-D3 alleles. PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of Glu-A3 and Glu-B3 alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels. Glu-D3c and Glu-D3e are the same allele. Two new alleles, namely, Glu-D3m in cultivar Darius, and Glu-D3n in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat.
536			_aGlobal Wheat Program
546			_aEnglish
593			_aLucia Segura
594			_aINT2411|INT0368
595			_aCSC
700	1		_aAili Wang, _ecoaut.
700	1		_91980 _aAppels, R.
700	1		_aBranlard, G., _ecoaut. _91976
700	1		_aHisashi Yoshida, _ecoaut.
700	1		_aKolman, M.A., _ecoaut.
700	1		_aLerner, S.E., _ecoaut. _91977
700	1		_aLinhai Wang, _ecoaut.
700	1		_aRogers, W.J., _ecoaut. _91975
700	1		_aTatsuya M Ikeda, _ecoaut.
700	1		_aWujun Ma, _ecoaut. _91979
700	1		_aXianchun Xia, _ecoaut. _9377
700	1		_aYueming Yan, _ecoaut.
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	1		_aPeña-Bautista, R.J. _8INT0368 _gGlobal Wheat Program _9645
942			_cPRO
999			_c8088 _d8088