000			02996naa a22003497a 4500
001			G94570
003			MX-TxCIM
005			20220708204441.0
008			220708s2010 mx ||||| |||| 10| 0 eng d
020			_a978-979-1159-41-8
040			_aMX-TxCIM
041			_aeng
090			_aCIS-6136
100	1		_aDutta, R. _928078
245	1	2	_aA simple and high throughput technique for field inoculation of Exserohilum turcicum in maize
260			_aMexico, DF (Mexico) : _bCIMMYT, _c2010.
520			_aTurcicum leaf blight (TLB) is a major disease that causes losses up to 98% in cool and humid conditions. Disease management depends upon resistant sources. However, nonavailability of a foolproof technique makes it difficult to locate resistant sources. Among the existing techniques, the hypodermic method is better but tedious, and causes injury to plants. To find a better alternative technique for inducing field infection, a modified treatment of actual lesion powder was compared with existing methods in glasshouse conditions with a susceptible control (CM 202). The treatment was tested in the field for refinement at a hot-spot location (Barapani, Meghalaya, India; 1000 m msl). Infected leaves were collected from a TLB-affected maize field and actual lesion area was collected. The collected portions of leaves were dried in the shade and pulverized. Two pinches of powder were applied in the central whorl at four growth stages from 2-3 to 5-6 leaf. Both single and repeated applications were tried with two susceptible controls, RCM 1-2 (local) and CM 202 and a resistant control, CM 104. Powder applied at various stages of growth had a significant difference. However, repeat application of powder had no significant difference from single application. Final disease data were recorded on an individual plant basis at the silk drying stage. Powder applied at the 3-4 leaf stage was found effective in inducing field infection abundantly, reaching the score of 5.0 (on a 1-5 rating scale). Hence, a single application of powder collected from the lesion area and applied to maize leaf whorls at the 3-4 leaf stage was found sufficient to induce a full field infection.
536			_aGlobal Maize Program
546			_aText in English
594			_aINT2823
650		7	_aInoculation _2AGROVOC _95194
650		7	_aTechniques _2AGROVOC _913203
650		7	_aSetosphaeria turcica _2AGROVOC _92254
650		7	_aMaize _2AGROVOC _91173
700	1		_aChandra, S. _921786
700	1		_aNgachan, S.V. _928080
700	1		_9862 _aZaidi, P.H. _gGlobal Maize Program _8INT2823
700	1		_aLal, S. _928081
711	2		_96553 _aAsian Regional Maize Workshop _n(10th : _dOctober 20-23, 2008 : _cMakassar, Indonesia)
773			_dMexico : CIMMYT, 2010. _gp. 416-419 _tAsian Regional Maize Workshop, 10. Maize for Asia - Emerging Trends and Technologies; Proceedings of The Asian Regional Maize Workshop; Makassar (Indonesia); 20-23 Oct. 2008 _wG94189 _z978-979-1159-41-8
942			_cBP _2ddc _n0
999			_c7989 _d7989