000			02786naa a22003497a 4500
001			G94566
003			MX-TxCIM
005			20220708183431.0
008			220708s2010 mx ||||| |||| 10| 0 eng d
020			_a978-979-1159-41-8
040			_aMX-TxCIM
041			_aeng
090			_aCIS-6131
100	1		_aTiwari, A. _924885
245	1	0	_aAllele mining in the Yellow1 (Y1) gene influencing carotenoid biosynthesis in maize
260			_aMexico, DF (Mexico) : _bCIMMYT, _c2010.
520			_aCarotenoids are a class of fat-soluble antioxidant vitamin compounds that provide health benefits to animals and humans. Four carotenoid compounds are predominant in maize grain: a- carotene, a-cryptoxanthin, zeaxanthin and lutein. The present study pertains to an analysis of 24 maize inbred lines out of an association mapping panel for nucleotide diversity in the Yellow1 (Y1) gene which regulates an important step in carotenoid biosynthesis in maize kernels. The 5 untranslated region (UTR) of the Y1 gene was specifically targeted for study since an earlier analysis had revealed that most of the variation associated with this gene is likely due to cis-acting regulatory sequences. Three gene-specific primer pairs for PCR were designed using PRIMER3 software based on the gene sequence available in the public domain. The PCR products were purified and quantified prior to DNA sequencing. Sequence ambiguities, if any, were resolved by repeated sequencing (at least three replicates) from both ends. All the sequences were analyzed using different programs of Bioedit software, leading to identification of several SNPs and indels. A total of 15 sequence variations have been found in the present study with a frequency of 1 SNP per 87 bp. Haplotyping and intensive phenotyping of the selected inbred lines is in progress for identification of favorable allelic variants for carotenoid biosynthesis in maize.
536			_aGenetic Resources Program|Global Maize Program
546			_aText in English
594			_aINT1617|INT3057
650		7	_aMaize _2AGROVOC _91173
650		7	_aGenes _2AGROVOC _93563
650		7	_aAlleles _2AGROVOC _92185
650		7	_aCarotenes _2AGROVOC _98110
650		7	_aRetinol _2AGROVOC _91241
700	1		_aPrasanna, B.M. _gGlobal Maize Program _8INT3057 _9887
700	1		_aGuru Prasad, K.N. _928071
700	1		_9832 _aPixley, K.V. _gGenetic Resources Program _8INT1617
711	2		_96553 _aAsian Regional Maize Workshop _n(10th : _dOctober 20-23, 2008 : _cMakassar, Indonesia)
773	0		_dMexico : CIMMYT, 2008. _gp. 224-227 _tAsian Regional Maize Workshop, 10. Maize for Asia - Emerging Trends and Technologies; Proceedings of The Asian Regional Maize Workshop; Makassar (Indonesia); 20-23 Oct. 2008 _wG94189 _z978-979-1159-41-8
942			_cBP _2ddc _n0
999			_c7985 _d7985